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SMAP-29
General Information
DCTPep ID DCTPep00001
Peptide Name SMAP-29
Sequence RGLRRLGRKIAHGVKKYGPTVLRIIRIA
Sequence Length 28
UniProt ID Not available
PubChem CID 16130512
Origin Ovis aries
Type Native peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=30 µM | MTT assay | 2 days | 2 |
Hemolytic Activity Human erythrocytes (4% Hemolysis=4 µM; 19% Hemolysis=20 µM; 67% Hemolysis=80 µM; 50% Hemolysis=86 µM; 10% Hemolysis=6.0 µm) ; Sheep erythrocytes (3% Hemolysis=80 µM)
Normal (non-cancerous) Cytotoxicity NIH 3T3: IC50=16 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00001
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C144H257N51O31
Absent amino acids CDEFMNQSW
Theoretical pI 12.31
Acidic residues 0
Basic residues 10
Polar residues 6
Molecular weight (Average) 3198.95
Molecular weight (Monoisotopic) 3197.01
Common amino acids R
Net charge 10
Instability index (II) 27
Aliphatic index 125.36
Grand average of hydropathicity (GRAVY) -0.204
Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.466
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 10601638
Title SMAP-29: a potent antibacterial and antifungal peptide from sheep leukocytes
Doi 10.1016/s0014-5793(99)01600-2
Year 1999
Literature 2
Pubmed ID 24221355
Title Bacterial killing mechanism of sheep myeloid antimicrobial peptide-18 (SMAP-18) and its Trp-substituted analog with improved cell selectivity and reduced mammalian cell toxicity
Year 2014
Literature 3
Pubmed ID 26795535
Title The stereochemical effect of SMAP-29 and SMAP-18 on bacterial selectivity, membrane interaction and anti-inflammatory activity
Year 2016
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DRAMP ID DRAMP02912
DBAASP ID 1485
This work is licensed under a Creative Commons Attribution 4.0 International License.