Peptide Libraray

CA-MA

General Information

DCTPep ID DCTPep00002

Peptide Name CA-MA

Sequence KWKLFKKIGIGKFLHSAKKF

Sequence Length 20

UniProt ID Not available

PubChem CID 16197683

Origin Ceropin-Magainin hybrid peptides

Type Synthetic peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
NCI-H146	Lung small cell carcinoma; Small cell lung cancer	IC50=15.8 µM	MTT assay	72 h	2
NCI-H69	Lung small cell carcinoma; Small cell lung cancer	IC50=15.8 µM	MTT assay	72 h	2
NCI-H128	Lung small cell carcinoma; Small cell lung cancer	IC50=16.1 µM	MTT assay	72 h	2
Jurkat	Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia	IC50=30 µM	MTT assay	3 days	1
MDA-MB-361	Breast adenocarcinoma	IC50=44 µM	MTT assay	3 days	1
A549	Lung adenocarcinoma	IC50=60 µM	MTT assay	3 days	1
K562	Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia	IC50=65 µM	MTT assay	3 days	1

Hemolytic Activity Human erythrocytes (Hemolysis 0% =100 μM)

Normal (non-cancerous) Cytotoxicity NIH-3T3 fibroblast: IC50>100 μM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID 1D9J

Predicted Structure DCTPep00002

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C120H190N30O22

Absent amino acids CDEMNPQRTVY

Theoretical pI 10.78

Acidic residues 0

Basic residues 8

Polar residues 3

Molecular weight (Average) 2405.02

Molecular weight (Monoisotopic) 2403.47

Common amino acids K

Net charge 8

Instability index (II) -15

Aliphatic index 83.00

Grand average of hydropathicity (GRAVY) -0.310

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.287

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 10675500

Title Effects of the hinge region of cecropin A(1-8)-magainin 2(1-12), a synthetic antimicrobial peptide, on liposomes, bacterial and tumor cells

Doi 10.1016/s0005-2736(99)00210-2

Year 2000

Literature 2

Pubmed ID 9352466

Title Structure-antitumor and hemolytic activity relationships of synthetic peptides derived from cecropin A-magainin 2 and cecropin A-melittin hybrid peptides

Doi 10.1111/j.1399-3011.1997.tb01469.x

Year 1997

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available