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CA-MA3
General Information
DCTPep ID DCTPep00003
Peptide Name CA-MA3
Sequence KWKLFKKIGPGKFLHSAKKF
Sequence Length 20
UniProt ID Not available
PubChem CID 16138048
Origin Ceropin-Magainin hybrid peptides
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| Jurkat | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | IC50=75 µM | MTT assay | 3 days | 1 |
| A549 | Lung adenocarcinoma | IC50>100 µM | MTT assay | 3 days | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | IC50>100 µM | MTT assay | 3 days | 1 |
| MDA-MB-361 | Breast adenocarcinoma | IC50>100 µM | MTT assay | 3 days | 1 |
Hemolytic Activity Human erythrocytes: Hemolysis 0% (100 μM)
Normal (non-cancerous) Cytotoxicity NIH-3T3 fibroblast: IC50>100 μM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00003
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C119H186N30O22
Absent amino acids CDEMNQRTVY
Theoretical pI 10.78
Acidic residues 0
Basic residues 8
Polar residues 3
Molecular weight (Average) 2388.97
Molecular weight (Monoisotopic) 2387.44
Common amino acids K
Net charge 8
Instability index (II) -10.75
Aliphatic index 63.50
Grand average of hydropathicity (GRAVY) -0.615
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.302
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 10675500
Title Effects of the hinge region of cecropin A(1-8)-magainin 2(1-12), a synthetic antimicrobial peptide, on liposomes, bacterial and tumor cells
Doi 10.1016/s0005-2736(99)00210-2
Year 2000
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.