Peptide Libraray

CA-MA2

General Information

DCTPep ID DCTPep00005

Peptide Name CA-MA2

Sequence KWKLFKKIPKFLHSAKKF

Sequence Length 18

UniProt ID Not available

PubChem CID Not available

Origin Ceropin-Magainin hybrid peptides

Type Synthetic peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
Jurkat	Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia	IC50=20 µM	MTT assay	3 days	1
A549	Lung adenocarcinoma	IC50=25 µM	MTT assay	3 days	1
MDA-MB-361	Breast adenocarcinoma	IC50=28 µM	MTT assay	3 days	1
K562	Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia	IC50=35.1 µM	MTT assay	3 days	1

Hemolytic Activity Human erythrocytes: Hemolysis 0% (100 μM)

Normal (non-cancerous) Cytotoxicity NIH-3T3 fibroblast: IC50>100 μM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID 1D9M

Predicted Structure DCTPep00005

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C115H180N28O20

Absent amino acids CDEGMNQRTVY

Theoretical pI 10.78

Acidic residues 0

Basic residues 8

Polar residues 1

Molecular weight (Average) 2274.87

Molecular weight (Monoisotopic) 2273.39

Common amino acids K

Net charge 8

Instability index (II) -9.94

Aliphatic index 70.56

Grand average of hydropathicity (GRAVY) -0.639

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.418

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 10675500

Title Effects of the hinge region of cecropin A(1-8)-magainin 2(1-12), a synthetic antimicrobial peptide, on liposomes, bacterial and tumor cells

Doi 10.1016/s0005-2736(99)00210-2

Year 2000

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available