Peptide Libraray

Aurein1.2

General Information

DCTPep ID DCTPep00007

Peptide Name Aurein1.2

Sequence GLFDIIKKIAESF

Sequence Length 13

UniProt ID Not available

PubChem CID Not available

Origin Southern bell frog

Type Native peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
Brain tumor cell (NCI)	Brain tumor	LC50=10 µM	Not available	Not available	1
Breast tumor cell (NCI)	Breast tumor	LC50=10 µM	Not available	Not available	1
Colon tumor cell (NCI)	Colon tumor	LC50=10 µM	Not available	Not available	1
Lung tumor cell (NCI)	Lung tumor	LC50=10 µM	Not available	Not available	1
Ovary tumor cell (NCI)	Ovary tumor	LC50=10 µM	Not available	Not available	1
Prostate tumor cell (NCI)	Prostate tumor	LC50=10 µM	Not available	Not available	1
Renal tumor cell (NCI)	Renal tumor	LC50=10 µM	Not available	Not available	1
Skin tumor cell (NCI)	Skin tumor	LC50=10 µM	Not available	Not available	1
Leukemia tumor cell (NCI)	Leukemia tumor	LC50=10-100 µM	Not available	Not available	1

Hemolytic Activity Not available

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Anticancer activity is due to penetration and disruption of the membranes of the cancer cells

Structure Information

PDB ID 1VM5

Predicted Structure DCTPep00007

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C71H113N15O19

Absent amino acids CHMNPQRTVWY

Theoretical pI 6.07

Acidic residues 2

Basic residues 2

Polar residues 2

Molecular weight (Average) 1480.77

Molecular weight (Monoisotopic) 1479.83

Common amino acids I

Net charge 0

Instability index (II) 20.48

Aliphatic index 127.69

Grand average of hydropathicity (GRAVY) 0.669

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 10951191

Title The antibiotic and anticancer active aurein peptides from the Australian Bell Frogs Litoria aurea and Litoria raniformis the solution structure of aurein 1.2

Doi 10.1046/j.1432-1327.2000.01536.x

Year 2000

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available

External Code

DBAASP ID DBAASPR_779