Aurein1.2

General Information


DCTPep ID  DCTPep00007

Peptide Name   Aurein1.2

Sequence  GLFDIIKKIAESF

Sequence Length  13

UniProt ID  Not available

PubChem CID  Not available

Origin  Southern bell frog

Type  Native peptide

Classification

  

ACP Tumor active peptide Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
Brain tumor cell (NCI) Brain tumor LC50=10 µM Not available Not available 1
Breast tumor cell (NCI) Breast tumor LC50=10 µM Not available Not available 1
Colon tumor cell (NCI) Colon tumor LC50=10 µM Not available Not available 1
Lung tumor cell (NCI) Lung tumor LC50=10 µM Not available Not available 1
Ovary tumor cell (NCI) Ovary tumor LC50=10 µM Not available Not available 1
Prostate tumor cell (NCI) Prostate tumor LC50=10 µM Not available Not available 1
Renal tumor cell (NCI) Renal tumor LC50=10 µM Not available Not available 1
Skin tumor cell (NCI) Skin tumor LC50=10 µM Not available Not available 1
Leukemia tumor cell (NCI) Leukemia tumor LC50=10-100 µM Not available Not available 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Anticancer activity is due to penetration and disruption of the membranes of the cancer cells



Structure Information


PDB ID  1VM5 

Predicted Structure  DCTPep00007

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C71H113N15O19

Absent amino acids  CHMNPQRTVWY

Theoretical pI  6.07

Acidic residues  2

Basic residues  2

Polar residues  2

Molecular weight (Average)  1480.77

Molecular weight (Monoisotopic)  1479.83

Common amino acids  I

Net charge  0

Instability index (II)  20.48

Aliphatic index  127.69

Grand average of hydropathicity (GRAVY)  0.669

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Should not be visible by UV spectrophotometry.

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 10951191

Title  The antibiotic and anticancer active aurein peptides from the Australian Bell Frogs Litoria aurea and Litoria raniformis the solution structure of aurein 1.2

Doi 10.1046/j.1432-1327.2000.01536.x

Year  2000

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPR_779

DCTPep is developed by Dr.Zheng's team.