Peptide Libraray

Hepcidin-25

General Information

DCTPep ID DCTPep00013

Peptide Name Hepcidin-25

Sequence DTHFPICIFCCGCCHRSKCGMCCKT

Sequence Length 25

UniProt ID Not available

PubChem CID 133065673

Origin Homo sapiens; Pan troglodytes; Pongo pygmaeus; Gorilla gorilla; Nomascus concolor; Hylobates lar

Type Native peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
K562	Blast phase chronic myelogenous leukemia, BCR-ABL15 positive; Chronic myeloid leukemia	26% Killing=30 µM	Trypan blue assay	15 h	1

Hemolytic Activity Not available

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00013

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Cyclic

Disulfide/Other Bond Cys7<--->Cys23; Cys10<--->Cys13; Cys11<--->Cys19; Cys14<--->Cys22

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C113H178N34O31S9

Absent amino acids AELNQVWY

Theoretical pI 8.22

Acidic residues 1

Basic residues 5

Polar residues 13

Molecular weight (Average) 2797.41

Molecular weight (Monoisotopic) 2795.09

Common amino acids C

Net charge 4

Instability index (II) 37.76

Aliphatic index 31.20

Grand average of hydropathicity (GRAVY) 0.388

Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 500
Abs 0.1% (=1 g/l) 0.179, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 11113131

Title Hepcidin, a urinary antimicrobial peptide synthesized in the liver

Doi 10.1074/jbc.M008922200

Year 2001

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available