Peptide Libraray

HNP-2

General Information

DCTPep ID DCTPep00023

Peptide Name HNP-2

Sequence CYCRIPACIAGERRYGTCIYQGRLWAFCC

Sequence Length 29

UniProt ID P59666 P59665 Q6EZE9 G3QPD5

PubChem CID 16130867

Origin Human alpha-defensins

Type Native peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
RCCs (A-498, Caki-2, 786-0, 769-P, ACHN, LE 9211-RCC, TW33, N43)	Renal Cell Carcinoma	At higher concentrations than 25 μg/ml, HNPs-1, -2, and -3 exerted cytotoxic effects on all tested RCC lines in an in vitro serum-free culture system (at lower concentrations they stimulated cell grow	WST-1 assay	40 h	1

Hemolytic Activity Not available

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00023

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Cyclic

Disulfide/Other Bond Cys1<--->Cys29; Cys9<--->Cys18; Cys8<--->Cys28

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C147H223N43O37S6

Absent amino acids DHKMNSV

Theoretical pI 8.67

Acidic residues 1

Basic residues 4

Polar residues 13

Molecular weight (Average) 3377.01

Molecular weight (Monoisotopic) 3374.52

Common amino acids C

Net charge 3

Instability index (II) 42.13

Aliphatic index 64.14

Grand average of hydropathicity (GRAVY) 0.248

Half Life
1.2 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 10345
Abs 0.1% (=1 g/l) 3.063, assuming all pairs of Cys residues form cystines
Ext. coefficient 9970
Abs 0.1% (=1 g/l) 2.952, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 11943716

Title Human alpha-defensins HNPs-1, -2, and -3 in renal cell carcinoma: influences on tumor cell proliferation

Doi 10.1016/s0002-9440(10)62558-8

Year 2002

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available

External Code

CancerPPD ID 4431