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P18
General Information
DCTPep ID DCTPep00026
Peptide Name P18
Sequence KWKFKKIPKFLHLAKKF
Sequence Length 17
UniProt ID Not available
PubChem CID Not available
Origin Ceropin A
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | IC50=3.5 µM | MTT assay | 3 days | 1 |
| Jurkat | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | IC50=4 µM | MTT assay | 3 days | 1 |
| MDA-MB-361 | Breast adenocarcinoma | IC50=8 µM | MTT assay | 3 days | 1 |
Hemolytic Activity Human erythrocytes: hemolysis: 0%=100 µM; hemolysis: 0%=50 µM; hemolysis: 0%=25.0 µM; hemolysis: 0%=12.5 µM; hemolysis: 0%=6.25 µM; hemolysis: 0%=3.125 µM; hemolysis: 0%=1.56 µM; hemolysis: 0%=0.78 µM
Normal (non-cancerous) Cytotoxicity NIH 3T3: IC50=75 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00026
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C112H175N27O18
Absent amino acids CDEGMNQRSTVY
Theoretical pI 10.78
Acidic residues 0
Basic residues 8
Polar residues 0
Molecular weight (Average) 2187.79
Molecular weight (Monoisotopic) 2186.36
Common amino acids K
Net charge 8
Instability index (II) -6.12
Aliphatic index 74.71
Grand average of hydropathicity (GRAVY) -0.629
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.514
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 12005420
Title Antibacterial, antitumor and hemolytic activities of alpha-helical antibiotic peptide, P18 and its analogs
Doi 10.1034/j.1399-3011.2001.00934.x
Year 2001
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.