Peptide Libraray

N-5L

General Information

DCTPep ID DCTPep00036

Peptide Name N-5L

Sequence WKKIPKFLHLLKKF

Sequence Length 14

UniProt ID Not available

PubChem CID Not available

Origin Ceropin A

Type Synthetic peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
K562	Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia	IC50=10 µM	MTT assay	3 days	1
Jurkat	Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia	IC50=14 µM	MTT assay	3 days	1
MDA-MB-361	Breast adenocarcinoma	IC50=17 µM	MTT assay	3 days	1

Hemolytic Activity Human erythrocytes: hemolysis: 9.4%=100 µM; hemolysis: 2.6%=50 µM; hemolysis: 0.2%=25.0 µM; hemolysis: 0%=12.5 µM; hemolysis: 0%=6.25 µM; hemolysis: 0%=3.125 µM; hemolysis: 0%=1.56 µM; hemolysis: 0%=0.78 µM

Normal (non-cancerous) Cytotoxicity NIH 3T3: IC50=50 µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00036

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C94H148N22O15

Absent amino acids ACDEGMNQRSTVY

Theoretical pI 10.60

Acidic residues 0

Basic residues 6

Polar residues 0

Molecular weight (Average) 1826.35

Molecular weight (Monoisotopic) 1825.15

Common amino acids K

Net charge 6

Instability index (II) -4.9

Aliphatic index 111.43

Grand average of hydropathicity (GRAVY) -0.264

Half Life
2.8 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 3.011

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 12005420

Title Antibacterial, antitumor and hemolytic activities of alpha-helical antibiotic peptide, P18 and its analogs

Doi 10.1034/j.1399-3011.2001.00934.x

Year 2001

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available