This work is licensed under a Creative Commons Attribution 4.0 International License.
Temporin-1Tl
General Information
DCTPep ID DCTPep00048
Peptide Name Temporin-1Tl
Sequence FVQWFSKFLGRIL
Sequence Length 13
UniProt ID Not available
PubChem CID 16139180
Origin Rana temporaria
Type Native peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| Hut-78 | Sezary syndrome | 100% Killing=10 µM | Trypan blue assay | Not available | 1 |
| U-937 | Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia | 20% Killing=10 µM | Trypan blue assay | Not available | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL9 positive; Chronic myeloid leukemia | 60% Killing=10 µM | Trypan blue assay | Not available | 1 |
Hemolytic Activity Human erythrocytes: 94% Hemolysis=24 µM; 92% Hemolysis=12 µM; 48% Hemolysis=6 µM; 13% Hemolysis=3 µM; 3% Hemolysis=1.5 µM; 100% Hemolysis=40 µM
Normal (non-cancerous) Cytotoxicity NIH 3T3: 100% Killing=40 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00048
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C83H121N19O16
Absent amino acids ACDEHMNPTY
Theoretical pI 11.00
Acidic residues 0
Basic residues 2
Polar residues 2
Molecular weight (Average) 1640.99
Molecular weight (Monoisotopic) 1639.92
Common amino acids F
Net charge 2
Instability index (II) 24.05
Aliphatic index 112.31
Grand average of hydropathicity (GRAVY) 0.823
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 3.352
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 12133008
Title Temporin L: antimicrobial, haemolytic and cytotoxic activities, and effects on membrane permeabilization in lipid vesicles
Year 2002
Literature 2
Pubmed ID 21319749
Title Structure-activity relationship, conformational and biological studies of temporin L analogues
Year 2011
Literature 3
Pubmed ID 32786286
Title A Noncytotoxic Temporin L Analogue with In Vivo Antibacterial and Antiendotoxin Activities and a Nonmembrane-Lytic Mode of Action
Doi 10.1021/acsinfecdis.0c00022
Year 2020
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.