Peptide Libraray

LFB 14-31 (L1)

General Information

DCTPep ID DCTPep00053

Peptide Name LFB 14-31 (L1)

Sequence PEWFKCRRWQWRMKKLGA

Sequence Length 18

UniProt ID P24627

PubChem CID Not available

Origin Bovine Lactoferrin

Type Native peptide

Classification

Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HT-29	Colon adenocarcinoma	IC50>404 µM	MTT assay	4 h	1
Meth A	Mouse fibrosarcoma	IC50>404 µM	MTT assay	4 h	1
MT-1	Human mammary carcinoma	IC50>404 µM	MTT assay	4 h	1

Hemolytic Activity Human red blood cells: EC50>404 µM

Normal (non-cancerous) Cytotoxicity Fibroblas: IC50>404 µM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00053

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C112H168N34O22S2

Absent amino acids DHINSTVY

Theoretical pI 11.01

Acidic residues 1

Basic residues 6

Polar residues 2

Molecular weight (Average) 2406.9

Molecular weight (Monoisotopic) 2405.25

Common amino acids KRW

Net charge 5

Instability index (II) 56.61

Aliphatic index 27.22

Grand average of hydropathicity (GRAVY) -1.339

Half Life
>20 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
? (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 16500
Abs 0.1% (=1 g/l) 6.855, assuming all pairs of Cys residues form cystines
Ext. coefficient 16500
Abs 0.1% (=1 g/l) 6.855, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 12366526

Title Enhanced antitumor activity and selectivity of lactoferrin-derived peptides

Doi 10.1034/j.1399-3011.2002.21008.x

Year 2002

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available