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L5
General Information
DCTPep ID DCTPep00057
Peptide Name L5
Sequence PAWRKAFRWAWRMLKKAA
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HT-29 | Colon adenocarcinoma | IC50=14 µM | MTT assay | 4 h | 1 |
| A549 | Lung adenocarcinoma | IC50=3 ± 1 μM(pH=7.4); IC50=4 ± 1 μM(pH=5.5) | MTT assay | 2 h | 2 |
| Meth A | Mouse fibrosarcoma | IC50=6.6 µM | MTT assay | 4 h | 1 |
| MT-1 | Human mammary carcinoma | IC50=8 µM | MTT assay | 4 h | 1 |
Hemolytic Activity Human red blood cells: EC50=33 µM
Normal (non-cancerous) Cytotoxicity Fibroblas: IC50=17 µM; CHO: IC50=5 ± 1 μM(pH=7.4); IC50=5 ± 1 μM(pH=5.5)
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00057
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C109H165N33O19S1
Absent amino acids CDEGHINQSTVY
Theoretical pI 12.31
Acidic residues 0
Basic residues 6
Polar residues 0
Molecular weight (Average) 2273.78
Molecular weight (Monoisotopic) 2272.27
Common amino acids A
Net charge 6
Instability index (II) 38.9
Aliphatic index 49.44
Grand average of hydropathicity (GRAVY) -0.667
Half Life
>20 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
? (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 16500
Abs 0.1% (=1 g/l) 7.257
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 12366526
Title Enhanced antitumor activity and selectivity of lactoferrin-derived peptides
Doi 10.1034/j.1399-3011.2002.21008.x
Year 2002
Literature 2
Pubmed ID 19464332
Title Constructing bioactive peptides with pH-dependent activities
Doi 10.1016/j.peptides.2009.05.009
Year 2009
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPS_4527
This work is licensed under a Creative Commons Attribution 4.0 International License.