Varv H

General Information


DCTPep ID  DCTPep00074

Peptide Name   Varv H

Sequence  GLPVCGETCFGGTCNTPGCSCETWPVCSRN

Sequence Length  30

UniProt ID  P58453 

PubChem CID  Not available

Origin  Viola arvensis (European field pansy) (Field violet)

Type  Native peptide

Classification

  

ACP Tumor active peptide Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
U251 Astrocytoma IC50=44.70 µg/ml SRB assay 48h 1
A549 Lung adenocarcinoma IC50>10 µg/ml SRB assay 48h 1
BEL-7402 Human papillomavirus-related endocervical adenocarcinoma IC50>10 µg/ml SRB assay 48h 1
DU145 Prostate carcinoma IC50>10 µg/ml SRB assay 48h 1
MDA-MB-231 Breast adenocarcinoma IC50>10 µg/ml SRB assay 48h 1

Hemolytic Activity  HRBCs: HD50=7.52 µM

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00074

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys27; NCB: Gly1<--->Asn30

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C125H192N36O43S6

Absent amino acids  ADHIKMQY

Theoretical pI  4.53

Acidic residues  2

Basic residues  1

Polar residues  19

Molecular weight (Average)  3079.47

Molecular weight (Monoisotopic)  3077.23

Common amino acids  C

Net charge  -1

Instability index (II)  41.26

Aliphatic index  32.33

Grand average of hydropathicity (GRAVY)  -0.020

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 5875
  Abs 0.1% (=1 g/l) 1.908, assuming all pairs of Cys residues form cystines
  Ext. coefficient 5500
  Abs 0.1% (=1 g/l) 1.786, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 20580652

Title  Isolation and characterization of cytotoxic cyclotides from Viola tricolor

Doi 10.1016/j.peptides.2010.05.004

Year  2010

Literature 2

Pubmed ID 10600388

Title  Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif

Doi 10.1006/jmbi.1999.3383

Year  1999

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.