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Varv H
General Information
DCTPep ID DCTPep00074
Peptide Name Varv H
Sequence GLPVCGETCFGGTCNTPGCSCETWPVCSRN
Sequence Length 30
UniProt ID P58453
PubChem CID Not available
Origin Viola arvensis (European field pansy) (Field violet)
Type Native peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| U251 | Astrocytoma | IC50=44.70 µg/ml | SRB assay | 48h | 1 |
| A549 | Lung adenocarcinoma | IC50>10 µg/ml | SRB assay | 48h | 1 |
| BEL-7402 | Human papillomavirus-related endocervical adenocarcinoma | IC50>10 µg/ml | SRB assay | 48h | 1 |
| DU145 | Prostate carcinoma | IC50>10 µg/ml | SRB assay | 48h | 1 |
| MDA-MB-231 | Breast adenocarcinoma | IC50>10 µg/ml | SRB assay | 48h | 1 |
Hemolytic Activity HRBCs: HD50=7.52 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00074
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys27; NCB: Gly1<--->Asn30
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C125H192N36O43S6
Absent amino acids ADHIKMQY
Theoretical pI 4.53
Acidic residues 2
Basic residues 1
Polar residues 19
Molecular weight (Average) 3079.47
Molecular weight (Monoisotopic) 3077.23
Common amino acids C
Net charge -1
Instability index (II) 41.26
Aliphatic index 32.33
Grand average of hydropathicity (GRAVY) -0.020
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5875
Abs 0.1% (=1 g/l) 1.908, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.786, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 20580652
Title Isolation and characterization of cytotoxic cyclotides from Viola tricolor
Doi 10.1016/j.peptides.2010.05.004
Year 2010
Literature 2
Pubmed ID 10600388
Title Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif
Year 1999
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.