This work is licensed under a Creative Commons Attribution 4.0 International License.
Citropin 1.1
General Information
DCTPep ID DCTPep00077
Peptide Name Citropin 1.1
Sequence GLFDVIKKVASVIGGL
Sequence Length 16
UniProt ID P81835
PubChem CID Not available
Origin Amphibian skin secretions
Type Native peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| U-937 | Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia | 60% Cytotoxicity at 0.5 µg/ml | MTT assay | 6 h | 2 |
| Breast cancer group (NCI) | Breast Carcinoma | IC50=10 μM | Not available | Not available | 1 |
| CNS cancer group (NCI) | CNS Carcinoma | IC50=10 μM | Not available | Not available | 1 |
| Colon cancer group (NCI) | Colon Carcinoma | IC50=10 μM | Not available | Not available | 1 |
| Leukemia group (NCI) | Leukemia | IC50=10 μM | Not available | Not available | 1 |
| Lung cancer group (NCI) | Lung Carcinoma | IC50=10 μM | Not available | Not available | 1 |
| Melanoma cancer group (NCI) | Melanoma cancer group (NCI) | IC50=10 μM | Not available | Not available | 1 |
| Ovarian cancer group (NCI) | Ovarian Carcinoma | IC50=10 μM | Not available | Not available | 1 |
| Prostate cancer group (NCI) | Prostate Carcinoma | IC50=10 μM | Not available | Not available | 1 |
| Renal cancer group (NCI) | Renal Carcinoma | IC50=10 μM | Not available | Not available | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00077
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C76H130N18O20
Absent amino acids CEHMNPQRTWY
Theoretical pI 8.59
Acidic residues 1
Basic residues 2
Polar residues 4
Molecular weight (Average) 1615.98
Molecular weight (Monoisotopic) 1614.97
Common amino acids GV
Net charge 1
Instability index (II) 14.19
Aliphatic index 158.12
Grand average of hydropathicity (GRAVY) 1.281
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Should not be visible by UV spectrophotometry.
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 12631273
Title nNOS inhibition, antimicrobial and anticancer activity of the amphibian skin peptide, citropin 1.1 and synthetic modifications
Doi 10.1046/j.1432-1033.2003.03462.x
Year 2003
Literature 2
Pubmed ID 21403917
Title Antitumor activity of antimicrobial peptides against U937 histiocytic cell line
Doi Not available
Year 2011
Literature 3
Pubmed ID 8573171
Title A novel antimicrobial peptide from Bufo bufo gargarizans
Year 1996
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.