This work is licensed under a Creative Commons Attribution 4.0 International License.
Magainin G
General Information
DCTPep ID DCTPep00097
Peptide Name Magainin G
Sequence GIGKFLHSAKKFAKAFVAEIMNS
Sequence Length 23
UniProt ID Not available
PubChem CID Not available
Origin African clawed frog Xenopus laevis skin
Type Native peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| NCI-H526 | Lung small cell carcinoma; Small cell lung cancer | IC50=10.5 µM | MTT assay | 24 h | 1 |
| NCI-H841 | Lung small cell carcinoma; Small cell lung cancer | IC50=11.7 µM | MTT assay | 24 h | 1 |
| NCI-H82 | Lung small cell carcinoma; Small cell lung cancer | IC50=12.5 µM | MTT assay | 24 h | 1 |
| RPMI-8402 | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | IC50=16 µg/ml | Trypan blue assay | 72 h | 3 |
| U-937 | Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia | IC50=19 µg/ml | Trypan blue assay | 72 h | 3 |
| Raji | EBV-related Burkitt lymphoma; Burkitt lymphoma | IC50=25 µg/ml | Trypan blue assay | 72 h | 3 |
| SSKT-1 | Leukemia | IC50=29 µg/ml | Trypan blue assay | 72 h | 3 |
| MLA | Leukemia | IC50=33 µg/ml | Trypan blue assay | 72 h | 3 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | IC50=37 µg/ml | Trypan blue assay | 72 h | 3 |
| Daudi | EBV-related Burkitt lymphoma; Burkitt lymphoma | IC50=38 µg/ml | Trypan blue assay | 72 h | 3 |
| CHP-100 | Ewing sarcoma | IC50=39 µg/ml | Trypan blue assay | 72 h | 3 |
| NCI-H678 | Lung small cell carcinoma; Small cell lung cancer | IC50=4.44 µM | MTT assay | 24 h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=42 µg/ml | Trypan blue assay | 72 h | 3 |
| PC-3 | Prostate carcinoma | IC50=47 µg/ml | Trypan blue assay | 72 h | 3 |
| NCI-H889 | Lung small cell carcinoma; Small cell lung cancer | IC50=6.57 µM | MTT assay | 24 h | 1 |
| NCI-H735 | Lung small cell carcinoma; Small cell lung cancer | IC50=7.23 µM | MTT assay | 24 h | 1 |
Hemolytic Activity Magainin G showed <1% hemolytic activity at 200 μg /ml (65.7μM)
Normal (non-cancerous) Cytotoxicity Magainin G did not exhibit cytotoxic effects on normal human peripheral blood lymphocytes at concentrations of up to 100-200 μg/ml (32.9-65.7 μM)
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00097
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Beta-alanine
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula Not Applicable
Absent amino acids Not Applicable
Theoretical pI Not Applicable
Acidic residues Not Applicable
Basic residues Not Applicable
Polar residues Not Applicable
Molecular weight (Average) 2494.98
Molecular weight (Monoisotopic) 2493.36
Common amino acids Not Applicable
Net charge Not Applicable
Instability index (II) Not Applicable
Aliphatic index Not Applicable
Grand average of hydropathicity (GRAVY) Not Applicable
Half Life
Not Applicable
Extinction coefficients
Not Applicable
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 1319823
Title Antitumor activity of magainin analogues against human lung cancer cell lines
Doi Not available
Year 1992
Literature 2
Pubmed ID 2285300
Title Magainin analogs effective against pathogenic protozoa
Year 1990
Literature 3
Pubmed ID 1708887
Title Antibiotic magainins exert cytolytic activi
Year
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.