This work is licensed under a Creative Commons Attribution 4.0 International License.
GGN6 (gaegurin 6)
General Information
DCTPep ID DCTPep00098
Peptide Name GGN6 (gaegurin 6)
Sequence FLPLLAGLAANFLPTIICKISYKC
Sequence Length 24
UniProt ID P80400
PubChem CID Not available
Origin Skin secretions of Rana rugosa
Type Native peptide
Classification
ACP Tumor active peptide Induce apoptosis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MCF-7 | Invasive breast carcinoma of no special type | IC50=4.88±0.53 µg/ml | MTT assay | 96 h | 1 |
| Hep 3B | Childhood hepatocellular carcinoma; Pediatric hepatocellular carcinoma | IC50=4.91±0.80 µg/ml | MTT assay | 96 h | 1 |
| PC-3 | Prostate carcinoma | IC50=5.75±1.17 µg/ml | MTT assay | 96 h | 1 |
| A549 | Lung adenocarcinoma | IC50=6.49 ± 1.39 µg/ml | MTT assay | 96 h | 1 |
Hemolytic Activity Human RBCs: less than 6% hemolysis at 100 μg/ml
Normal (non-cancerous) Cytotoxicity HEK293: IC50=5.62±1.50 µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00098
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys18<--->Cys24
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C125H201N27O29S2
Absent amino acids DEHMQRVW
Theoretical pI 8.86
Acidic residues 0
Basic residues 2
Polar residues 7
Molecular weight (Average) 2610.25
Molecular weight (Monoisotopic) 2608.45
Common amino acids L
Net charge 2
Instability index (II) 15.83
Aliphatic index 142.50
Grand average of hydropathicity (GRAVY) 1.283
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 1615
Abs 0.1% (=1 g/l) 0.619, assuming all pairs of Cys residues form cystines
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.571, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 14499271
Title In vitro activities of native and designed peptide antibiotics against drug sensitive and resistant tumor cell lines
Doi 10.1016/s0196-9781(03)00194-3
Year 2003
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
This work is licensed under a Creative Commons Attribution 4.0 International License.