Peptide Libraray

PTP1

General Information

DCTPep ID DCTPep00099

Peptide Name PTP1

Sequence LLAGLAANFLPTIICKISYKC

Sequence Length 21

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

Induce apoptosis

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
A549	Lung adenocarcinoma	IC50>100 µg/ml	MTT assay	96 h	1
Hep 3B	Childhood hepatocellular carcinoma; Pediatric hepatocellular carcinoma	IC50>100 µg/ml	MTT assay	96 h	1
MCF-7	Invasive breast carcinoma of no special type	IC50>100 µg/ml	MTT assay	96 h	1
PC-3	Prostate carcinoma	IC50>100 µg/ml	MTT assay	96 h	1

Hemolytic Activity Human RBCs: less than 6% hemolysis at 100 μg/ml

Normal (non-cancerous) Cytotoxicity HEK293: IC50>100 µg/ml

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00099

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Cyclic

Disulfide/Other Bond Cys15<--->Cys21

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C105H174N24O26S2

Absent amino acids DEHMQRVW

Theoretical pI 8.86

Acidic residues 0

Basic residues 2

Polar residues 7

Molecular weight (Average) 2252.8

Molecular weight (Monoisotopic) 2251.25

Common amino acids L

Net charge 2

Instability index (II) 7.5

Aliphatic index 144.29

Grand average of hydropathicity (GRAVY) 1.229

Half Life
5.5 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 1615
Abs 0.1% (=1 g/l) 0.717, assuming all pairs of Cys residues form cystines
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.661, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 14499271

Title In vitro activities of native and designed peptide antibiotics against drug sensitive and resistant tumor cell lines

Doi 10.1016/s0196-9781(03)00194-3

Year 2003

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available