PTP4

General Information


DCTPep ID  DCTPep00101

Peptide Name   PTP4

Sequence  FLKLLKKLAAKFLPTIICKISYKC

Sequence Length  24

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Induce apoptosis



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
A549 Lung adenocarcinoma IC50=13.71 ± 2.12 µg/ml MTT assay 96 h 1
MCF-7 Invasive breast carcinoma of no special type IC50=18.10 ± 2.13 µg/ml MTT assay 96 h 1
Hep 3B Childhood hepatocellular carcinoma; Pediatric hepatocellular carcinoma IC50=18.18 ± 3.31 µg/ml MTT assay 96 h 1
PC-3 Prostate carcinoma IC50=18.63 ± 0.99 µg/ml MTT assay 96 h 1

Hemolytic Activity  Human RBCs: 45.17% hemolysis at 100 μg/ml

Normal (non-cancerous) Cytotoxicity  HEK293: IC50=26.15 ± 6.23 µg/ml

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00101

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C135H228N30O28S2

Absent amino acids  DEGHMNQRVW

Theoretical pI  9.93

Acidic residues  0

Basic residues  6

Polar residues  5

Molecular weight (Average)  2783.6

Molecular weight (Monoisotopic)  2781.68

Common amino acids  K

Net charge  6

Instability index (II)  -0.08

Aliphatic index  138.33

Grand average of hydropathicity (GRAVY)  0.787

Half Life 
  1.1 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 1615
  Abs 0.1% (=1 g/l) 0.580, assuming all pairs of Cys residues form cystines
  Ext. coefficient 1490
  Abs 0.1% (=1 g/l) 0.535, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 14499271

Title  In vitro activities of native and designed peptide antibiotics against drug sensitive and resistant tumor cell lines

Doi 10.1016/s0196-9781(03)00194-3

Year  2003

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




CancerPPD ID  1004

CancerPPD ID  1012

CancerPPD ID  1020

CancerPPD ID  1028

CancerPPD ID  1036

DCTPep is developed by Dr.Zheng's team.