This work is licensed under a Creative Commons Attribution 4.0 International License.
CIGB-300 (P15-Tat)
General Information
DCTPep ID DCTPep00119
Peptide Name CIGB-300 (P15-Tat)
Sequence CWMSPRHLGTCGRKKRRQRRRPPQ
Sequence Length 24
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide Transducing peptide Cell-penetrating peptides
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| SiHa | Human papillomavirus-related cervical squamous cell carcinoma; Squamous cell carcinoma of the cervix uteri | IC50=135±10 µmol/L | MTS assay | 72 h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=136±16 µmol/L | MTS assay | 72 h | 1 |
| 3LL | Malignant tumors of the mouse pulmonary system | IC50=143±4 µmol/L | MTT assay | 72 h | 2 |
| A549 | Lung adenocarcinoma | IC50=171±12 µmol/L | MTT assay | 72 h | 2 |
| Ca Ski | Human papillomavirus-related cervical squamous cell carcinoma; Squamous cell carcinoma of the cervix uteri | IC50=182±6 µmol/L | MTS assay | 72 h | 1 |
| NCI-H82 | Lung small cell carcinoma; Small cell lung cancer | IC50=20±3 µmol/L | MTS assay | 72 h | 1 |
| PC-3 | Prostate carcinoma | IC50=201±16 µmol/L | MTT assay | 72 h | 2 |
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | IC50=30±3 µmol/L | MTT assay | 72 h | 2 |
| NCI-H125 | Lung adenosquamous carcinoma | IC50=36±9 µmol/L | MTS assay | 72 h | 1 |
| TC-1 | Human renal cell carcinoma | IC50=71±15 µmol/L | MTS assay | 72 h | 1 |
| Jurkat | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | IC50=89±3 µmol/L | MTS assay | 72 h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism CIGB-300 is a cyclic synthetic peptide that induces apoptosis in malignant cells, elicits antitumor activity in cancer animal models
Structure Information
PDB ID Not available
Predicted Structure DCTPep00119
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys1<--->Cys11
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C124H212N52O29S3
Absent amino acids ADEFINVY
Theoretical pI 12.13
Acidic residues 0
Basic residues 10
Polar residues 6
Molecular weight (Average) 2991.56
Molecular weight (Monoisotopic) 2989.59
Common amino acids R
Net charge 10
Instability index (II) 184.56
Aliphatic index 16.25
Grand average of hydropathicity (GRAVY) -1.950
Half Life
1.2 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5625
Abs 0.1% (=1 g/l) 1.880, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.839, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 15466209
Title Antitumor effect of a novel proapoptotic peptide that impairs the phosphorylation by the protein kinase 2 (casein kinase 2)
Doi 10.1158/0008-5472.CAN-04-2086
Year 2004
Literature 2
Pubmed ID 24773585
Title Mechanisms of cellular uptake, intracellular transportation, and degradation of CIGB-300, a Tat-conjugated peptide, in tumor cell lines
Year 2014
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.