This work is licensed under a Creative Commons Attribution 4.0 International License.
TAT-Ras-GAP317-326
General Information
DCTPep ID DCTPep00120
Peptide Name TAT-Ras-GAP317-326
Sequence GRKKRRQRRRGGWMWVTNLRTD
Sequence Length 22
UniProt ID Not available
PubChem CID Not available
Origin RasGAP-derived peptides
Type Native peptide
Classification
Transducing peptide Cell-penetrating peptides
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | rendered all the tested cancer cell lines sensitive to doses of cisplatin, adriamycin, and mitoxantrone | FITC fluorophore assay | 20 h | 1 |
| H-Meso-1 | Human malignant mesothelomia | rendered all the tested cancer cell lines sensitive to doses of cisplatin, adriamycin, and mitoxantrone | FITC fluorophore assay | 20 h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | rendered all the tested cancer cell lines sensitive to doses of cisplatin, adriamycin, and mitoxantrone | FITC fluorophore assay | 20 h | 1 |
| U2OS | Human osteosarcoma | rendered all the tested cancer cell lines sensitive to doses of cisplatin, adriamycin, and mitoxantrone | FITC fluorophore assay | 20 h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Enhances genotoxin-induced cytotoxicity in tumor cells
Structure Information
PDB ID Not available
Predicted Structure DCTPep00120
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C119H201N49O29S1
Absent amino acids ACEFHIPSY
Theoretical pI 12.40
Acidic residues 1
Basic residues 9
Polar residues 6
Molecular weight (Average) 2814.28
Molecular weight (Monoisotopic) 2812.55
Common amino acids R
Net charge 8
Instability index (II) 123.55
Aliphatic index 30.91
Grand average of hydropathicity (GRAVY) -2.014
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 11000
Abs 0.1% (=1 g/l) 3.909
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 15467750
Title A RasGAP-derived cell permeable peptide potently enhances genotoxin-induced cytotoxicity in tumor cells
Year 2004
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.