Peptide Libraray

Apo E

General Information

DCTPep ID DCTPep00124

Peptide Name Apo E

Sequence LRVRLASHLRKLRKRLLRDADDLQKRLAVY

Sequence Length 30

UniProt ID Not available

PubChem CID Not available

Origin Apoprotein E

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
MKN7	Gastric tubular adenocarcinoma	35 % Cytotoxity at 40 µg/ml	MTT assay	4 h	1
Mia PaCa-2	Pancreatic ductal adenocarcinoma	55 % Cytotoxity at 40 µg/ml	MTT assay	4 h	1
PANC-1	Pancreatic ductal adenocarcinoma	55 % Cytotoxity at 40 µg/ml	MTT assay	4 h	1
MKN1	Gastric adenosquamous carcinoma	60 % Cytotoxity at 40 µg/ml	MTT assay	4 h	1
COLO 201	Colon adenocarcinoma	65 % Cytotoxity at 40 µg/ml	MTT assay	4 h	1

Hemolytic Activity Human red blood cells: ApoE does not lyse erythrocytes

Normal (non-cancerous) Cytotoxicity Not available

Target Not available

Affinity Not available

Mechanism ApoE has anti-tumor activity, probably through perturbation and formation of ionpermeable "pores" in membranes

Structure Information

PDB ID Not available

Predicted Structure DCTPep00124

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C162H287N57O40

Absent amino acids CEFGIMNPTW

Theoretical pI 11.63

Acidic residues 3

Basic residues 11

Polar residues 2

Molecular weight (Average) 3673.42

Molecular weight (Monoisotopic) 3671.22

Common amino acids L

Net charge 8

Instability index (II) 59.77

Aliphatic index 133.33

Grand average of hydropathicity (GRAVY) -0.610

Half Life
5.5 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.406

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 15796184

Title Anti-tumor activity of an antibiotic peptide derived from apoprotein E

Doi Not available

Year 2005

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available