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Pep27anal2
General Information
DCTPep ID DCTPep00138
Peptide Name Pep27anal2
Sequence MWKWFHNVLSWWWLLADKRPARDYNRK
Sequence Length 27
UniProt ID Not available
PubChem CID Not available
Origin S. pneumoniae
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MCF-7 | Invasive breast carcinoma of no special type | IC50<10 µM | MTT assay | 22.5 min | 1 |
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | IC50=20 µM | MTT assay | 22.5 min | 1 |
| Jurkat | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | IC50=23 µM | MTT assay | 22.5 min | 1 |
| SNU-601 | Gastric signet ring cell adenocarcinoma | IC50=25 µM | MTT assay | 22.5 min | 1 |
| OCI-AML-2 | Adult acute myeloid leukemia; Acute myeloid leukemia | IC50=29 µM | MTT assay | 22.5 min | 1 |
Hemolytic Activity Human RBCs(18% hemolysis at 12.5 μM), Human RBCs(0% hemolysis at 0.19 μM, 0.39 μM, 0.78 μM, 1.56 μM, 3.12 μM, 6.25 μM)
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Pep27anal2 can penetrate the plasma membrane, and then induce apoptosis in both caspase-and cytochrome c-independent manner
Structure Information
PDB ID Not available
Predicted Structure DCTPep00138
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C173H244N48O36S1
Absent amino acids CEGIQT
Theoretical pI 10.43
Acidic residues 2
Basic residues 7
Polar residues 4
Molecular weight (Average) 3604.2
Molecular weight (Monoisotopic) 3601.85
Common amino acids W
Net charge 5
Instability index (II) 43.02
Aliphatic index 61.48
Grand average of hydropathicity (GRAVY) -0.989
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 28990
Abs 0.1% (=1 g/l) 8.043
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 16004618
Title Functional and structural characteristics of anticancer peptide Pep27 analogues
Year 2005
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.