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MPI
General Information
DCTPep ID DCTPep00146
Peptide Name MPI
Sequence IDWKKLLDAAKQIL
Sequence Length 14
UniProt ID Not available
PubChem CID Not available
Origin Polybia paulista
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| PC-3 | Prostate carcinoma | IC50=23.7±2.9 µM | MTT assay | 24h | 1 |
| HepG2 | Hepatoblastoma | IC50=25.2±1.8 µM | MTT assay | 24h | 1 |
| EJ | Endometrial Adenocarcinoma | IC50=25±1.9 µM | MTT assay | 24h | 1 |
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL10 positive; Chronic myeloid leukemia | IC50=5.06±0.1 µM | MTT assay | 24h | 1 |
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | IC50=5.86±0.18 µM | MTT assay | 24h | 1 |
| L1210 | Mouse leukemia | IC50=6.18±0.53 µM | MTT assay | 24h | 1 |
| K562/ADM | Blast phase chronic myelogenous leukemia, BCR-ABL17 positive; Chronic myeloid leukemia | IC50=7.01±1.4 µM | MTT assay | 24h | 1 |
Hemolytic Activity Human erythrocytes: Not active up to 64 µM; 3.2% Hemolysis=25 µM; <1% Hemolysis=12.5 µM
Normal (non-cancerous) Cytotoxicity Rat mast cells: 62% degranulation=100 µM; NIH 3T3: 50% Cell death>60 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00146
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C78H131N19O20
Absent amino acids CEFGHMNPRSTVY
Theoretical pI 8.50
Acidic residues 2
Basic residues 3
Polar residues 0
Molecular weight (Average) 1655.01
Molecular weight (Monoisotopic) 1653.98
Common amino acids KL
Net charge 1
Instability index (II) 33.89
Aliphatic index 153.57
Grand average of hydropathicity (GRAVY) 0.064
Half Life
20 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 3.323
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 19233550
Title Novel mode of action of polybia-MPI, a novel antimicrobial peptide, in multi-drug resistant leukemic cells
Doi 10.1016/j.canlet.2008.12.027
Year 2009
Literature 2
Pubmed ID 16129513
Title Structural and functional characterization of two novel peptide toxins isolated from the venom of the social wasp Polybia paulista
Doi 10.1016/j.peptides.2005.04.026
Year 2005
Literature 3
Pubmed ID 20600424
Title A novel analog of antimicrobial peptide Polybia-MPI, with thioamide bond substitution, exhibits increased therapeutic efficacy against cancer and diminished toxicity in mice
Doi 10.1016/j.peptides.2010.06.019
Year 2010
Literature 4
Pubmed ID 27900727
Title Antimicrobial activity and stability of the D-amino acid substituted derivatives of antimicrobial peptide polybia-MPI
Year 2016
Literature 5
Pubmed ID 29075946
Title Antimicrobial activity and stability of stapled helices of polybia-MP1
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.