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NK-2
General Information
DCTPep ID DCTPep00147
Peptide Name NK-2
Sequence KILRGVCKKIMRTFLRRISKDILTGKK
Sequence Length 27
UniProt ID Q29075
PubChem CID Not available
Origin Sus scrofa (Pig)
Type Native peptide
Classification
ACP Tumor active peptide Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | 28.7 ± 9.3% Cytotoxicity=100 μg/ml | MTT assay | 24 h | 3 |
| E42/02 | Horse sarcoid | IC50=~40 µM | MTT assay | 24 h | 2 |
| E42/02 | Horse sarcoid | IC50=~50 µM | MTT assay | 2 h | 2 |
| E42/02 | Horse sarcoid | IC50=~50 µM | MTT assay | 4 h | 2 |
| E42/02 | Horse sarcoid | IC50=~70 µM | MTT assay | 30 min | 2 |
| LA-N-1 | Neuroblastoma | LD50=1-4 µM | PI-uptake assay | Not available | 1 |
| SH-SY5Y | Neuroblastoma | LD50=1-4 µM | PI-uptake assay | Not available | 1 |
| SW480 | Colon adenocarcinoma | LD50=1-4 µM | PI-uptake assay | Not available | 1 |
| U-937 | Adult acute monocytic leukemi; Acute monoblastic/monocytic leukemia | LD50=30 µM | PI-uptake assay | Not available | 1 |
Hemolytic Activity Human erythrocytes: 45% Hemolysis=100 µg/ml
Normal (non-cancerous) Cytotoxicity Non-toxic to a human keratinocyte cell line
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00147
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C143H261N45O33S2
Absent amino acids AEHNPQWY
Theoretical pI 11.60
Acidic residues 1
Basic residues 10
Polar residues 6
Molecular weight (Average) 3203.05
Molecular weight (Monoisotopic) 3200.96
Common amino acids K
Net charge 9
Instability index (II) 45.43
Aliphatic index 111.85
Grand average of hydropathicity (GRAVY) -0.263
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 16269280
Title The NK-lysin derived peptide NK-2 preferentially kills cancer cells with increased surface levels of negatively charged phosphatidylserine
Doi 10.1016/j.febslet.2005.09.084
Year 2005
Literature 2
Pubmed ID 23893605
Title Design of NK-2-derived peptides with improved activity against equine sarcoid cells
Doi 10.1002/psc.2540
Year 2013
Literature 3
Pubmed ID 17389605
Title Rationale for the design of shortened derivatives of the NK-lysin-derived antimicrobial peptide NK-2 with improved activity against Gram-negative pathogens
Year 2007
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.