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Lactoferricin B
General Information
DCTPep ID DCTPep00153
Peptide Name Lactoferricin B
Sequence FKCRRWQWRMKKLGAPSITCVRRAF
Sequence Length 25
UniProt ID P24627
PubChem CID Not available
Origin The big centipede Scolopendra subspinipes mutilans
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| Kelly | Neuroblastoma | IC50=15.5 µM | MTT assay | 48 h | 1 |
| IMR-32 | Neuroblastoma | IC50=29 µM | MTT assay | 48 h | 1 |
| SK-N-DZ | Neuroblastoma | IC50=37 µM | MTT assay | 48 h | 1 |
| SHEP-12 | Neuroblastoma | IC50=45 µM | MTT assay | 48 h | 1 |
| SH-SY5Y | Neuroblastoma | IC50=60 µM | MTT assay | 48 h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism LfcinB possesses a cytotoxic effect on MYCN-amplified NB cells by directly targeting and destabilizing the cytoplasmic membrane and the mitochondria.
Structure Information
PDB ID 1LFC
Predicted Structure DCTPep00153
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3 <--> Cys20
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C141H226N46O29S3
Absent amino acids DEHNY
Theoretical pI 11.84
Acidic residues 0
Basic residues 8
Polar residues 5
Molecular weight (Average) 3125.82
Molecular weight (Monoisotopic) 3123.68
Common amino acids R
Net charge 8
Instability index (II) 77.92
Aliphatic index 50.80
Grand average of hydropathicity (GRAVY) -0.576
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 11125
Abs 0.1% (=1 g/l) 3.559, assuming all pairs of Cys residues form cystines
Ext. coefficient 11000
Abs 0.1% (=1 g/l) 3.519, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 16572423
Title The antimicrobial peptide, lactoferricin B, is cytotoxic to neuroblastoma cells in vitro and inhibits xenograft growth in vivo
Year 2006
Literature 2
Pubmed ID 11908643
Title Towards a structure-function analysis of bovine lactoferricin and related tryptophan- and arginine-containing peptides
Doi 10.1139/o01-213
Year 2002
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.