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Gaegurin-5
General Information
DCTPep ID DCTPep00167
Peptide Name Gaegurin-5
Sequence FLGALFKVASKVLPSVKCAITKKC
Sequence Length 24
UniProt ID Not available
PubChem CID Not available
Origin Rana rugosa
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HCT 116 | Colon carcinoma | IC50=113.31 µg/ml | MTT assay | 3 days | 1 |
| A498 | Renal cell carcinoma | IC50=138.06 µg/ml | MTT assay | 3 days | 1 |
| A549 | Lung adenocarcinoma | IC50=145.72 µg/ml | MTT assay | 3 days | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=183.74 µg/ml | MTT assay | 3 days | 1 |
| MKN 45 | Gastric adenocarcinoma | IC50=34.96 µg/ml | MTT assay | 3 days | 1 |
| SK-OV-3 | Ovarian serous cystadenocarcinoma | IC50=38.28 µg/ml | MTT assay | 3 days | 1 |
| NCI-H630 | Rectal carcinoma | IC50=41.85 µg/ml | MTT assay | 3 days | 1 |
| PC-3 | Prostate carcinoma | IC50=43.64 µg/ml | MTT assay | 3 days | 1 |
| SK-MEL-2 | Melanoma | IC50=47.47 µg/ml | MTT assay | 3 days | 1 |
Hemolytic Activity Human erythrocytes: 7% Hemolysis=100 µg/ml
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00167
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Cys18<--->Cys24
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C119H203N29O28S2
Absent amino acids DEHMNQRWY
Theoretical pI 9.90
Acidic residues 0
Basic residues 5
Polar residues 6
Molecular weight (Average) 2552.22
Molecular weight (Monoisotopic) 2550.48
Common amino acids K
Net charge 5
Instability index (II) 7.56
Aliphatic index 113.75
Grand average of hydropathicity (GRAVY) 0.863
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.049, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 16884301
Title Structural determinants for the membrane interaction of novel bioactive undecapeptides derived from gaegurin 5
Year 2006
Literature 2
Pubmed ID 14739294
Title Systematic peptide engineering and structural characterization to search for the shortest antimicrobial peptide analogue of gaegurin 5
Year 2004
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.