This work is licensed under a Creative Commons Attribution 4.0 International License.
Gramicidin S
General Information
DCTPep ID DCTPep00183
Peptide Name Gramicidin S
Sequence VXLfPVXLfP
Sequence Length 10
UniProt ID Not available
PubChem CID 73357
Origin Bacillus brevis
Type Native peptide
Classification
ACP Tumor active peptide Membrane lysis
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | LC50=7.4 µM | Resazurin assay | 24h | 1 |
| A549 | Lung adenocarcinoma | LC50=7.5 µM | Resazurin assay | 24h | 1 |
Hemolytic Activity Human erythrocytes: 100% Hemolysis=39 µg/ml; 50% Hemolysis=11 µM; Sheep erythrocytes: 50% Hemolysis=21.1±2.6 µM; 100% Hemolysis=100 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure Not available
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond NCB: Val1<--->Pro10
N-terminal Modification Free
C-terminal Modification Free
Other Modification X=Orn; f=D-Phe
Chiral Mix
Physicochemical Information
Formula Not Applicable
Absent amino acids Not Applicable
Theoretical pI Not Applicable
Acidic residues Not Applicable
Basic residues Not Applicable
Polar residues Not Applicable
Molecular weight (Average) Not Applicable
Molecular weight (Monoisotopic) Not Applicable
Common amino acids Not Applicable
Net charge Not Applicable
Instability index (II) Not Applicable
Aliphatic index Not Applicable
Grand average of hydropathicity (GRAVY) Not Applicable
Half Life
Not Applicable
Extinction coefficients
Not Applicable
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 17462586
Title Inhibition of malaria parasite blood stages by tyrocidines, membrane-active cyclic peptide antibiotics from Bacillus brevis
Doi 10.1016/j.bbamem.2007.01.015
Year 2007
Literature 2
Pubmed ID 8810288
Title Modulation of structure and antibacterial and hemolytic activity by ring size in cyclic gramicidin S analogs
Year 1996
Literature 3
Pubmed ID 19132829
Title Therapeutic index of gramicidin S is strongly modulated by D-phenylalanine analogues at the beta-turn
Year 2009
Literature 4
Pubmed ID 22976321
Title Polycationic gramicidin S analogues with both high antibiotic activity and very low hemolytic activity
Year 2012
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
External Code
DBAASP ID DBAASPN_1688
This work is licensed under a Creative Commons Attribution 4.0 International License.