This work is licensed under a Creative Commons Attribution 4.0 International License.
Arenicin-1
General Information
DCTPep ID DCTPep00187
Peptide Name Arenicin-1
Sequence RWCVYAYVRVRGVLVRYRRCW
Sequence Length 21
UniProt ID Not available
PubChem CID Not available
Origin Arenicola marina
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL11 positive; Chronic myeloid leukemia | IC50=17.9 µM | MTT assay | 24h | 2 |
| Jurkat | Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia | LD50=4 µM | Alamar blue assay | 30 min | 1 |
Hemolytic Activity Human erythrocytes: 0% Hemolysis=12.5 µM; 12% Hemolysis=25 µM; 35% Hemolysis=50 µM; 51% Hemolysis=100 µM; 10% Hemolysis=4.1 µM; 15% Hemolysis=10.3 µM; 50% Hemolysis=66.3 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00187
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys3<--->Cys20
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C127H195N41O25S2
Absent amino acids DEFHIKMNPQST
Theoretical pI 10.85
Acidic residues 0
Basic residues 6
Polar residues 6
Molecular weight (Average) 2760.33
Molecular weight (Monoisotopic) 2758.47
Common amino acids R
Net charge 6
Instability index (II) 63.76
Aliphatic index 92.38
Grand average of hydropathicity (GRAVY) -0.071
Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 15595
Abs 0.1% (=1 g/l) 5.650, assuming all pairs of Cys residues form cystines
Ext. coefficient 15470
Abs 0.1% (=1 g/l) 5.604, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 17935487
Title Structure and mode of action of the antimicrobial peptide arenicin
Year 2008
Literature 2
Pubmed ID 31234579
Title Redesigning Arenicin-1, an Antimicrobial Peptide from the Marine Polychaeta Arenicola marina, by Strand Rearrangement or Branching, Substitution of Specific Residues, and Backbone Linearization or Cyclization
Year 2019
Literature 3
Pubmed ID 21241661
Title The characteristic region of arenicin-1 involved with a bacterial membrane targeting mechanism
Doi 10.1016/j.bbrc.2011.01.046
Year 2011
Literature 4
Pubmed ID 25557880
Title Design of antimicrobial peptide arenicin analogs with improved therapeutic indices
Doi 10.1002/psc.2732
Year 2015
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.