Peptide Libraray

Tat-a5

General Information

DCTPep ID DCTPep00199

Peptide Name Tat-a5

Sequence KAQIRAMECNILGRKKRRQRRR

Sequence Length 22

UniProt ID Not available

PubChem CID Not available

Origin HIV-Tat (49-57)

Type Synthetic peptide

Classification

ACP Tumor active peptide Cancer targeted peptides

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HCT 116	Colon carcinoma	~80% Cytotoxicity at 100 µM	MTT assay	24 h	1

Hemolytic Activity Not available

Normal (non-cancerous) Cytotoxicity Not available

Target HIV-Tat

Affinity Not available

Mechanism Tat-a5 could arrest cancer cells at G2/M phase and make them apoptotic; Tat-a5 would have the potential to inhibit tumor angiogenesis

Structure Information

PDB ID Not available

Predicted Structure DCTPep00199

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C113H211N49O28S2

Absent amino acids DFHPSTVWY

Theoretical pI 12.13

Acidic residues 1

Basic residues 10

Polar residues 3

Molecular weight (Average) 2768.35

Molecular weight (Monoisotopic) 2766.6

Common amino acids R

Net charge 9

Instability index (II) 159.93

Aliphatic index 62.27

Grand average of hydropathicity (GRAVY) -1.673

Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 18656352

Title A novel peptide from alpha5 helix of Asterina pectinifera cyclin B conjugated to HIV-Tat(49-59) with cytotoxic and apoptotic effects against human cancer cells

Doi 10.1016/j.bmcl.2008.07.017

Year 2008

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available