This work is licensed under a Creative Commons Attribution 4.0 International License.
Crotamine (1–9,38–42)
General Information
DCTPep ID DCTPep00204
Peptide Name Crotamine (1–9,38–42)
Sequence YKQCHKKGGKKGSG
Sequence Length 14
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
Cell-penetrating peptides
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | Not active up to 100 µM | MTT assay | 4h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis>300µM
Normal (non-cancerous) Cytotoxicity PBMC: IC50>300µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00204
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C64H108N22O18S1
Absent amino acids ADEFILMNPRTVW
Theoretical pI 10.03
Acidic residues 0
Basic residues 6
Polar residues 7
Molecular weight (Average) 1505.76
Molecular weight (Monoisotopic) 1504.79
Common amino acids K
Net charge 6
Instability index (II) 51.64
Aliphatic index 0.00
Grand average of hydropathicity (GRAVY) -1.957
Half Life
2.8 hours (mammalian reticulocytes, in vitro).
10 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.990, assuming all pairs of Cys residues form cystines
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.990, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 18983137
Title A novel cell-penetrating peptide sequence derived by structural minimization of a snake toxin exhibits preferential nucleolar localization
Year 2008
Literature 2
Pubmed ID 28314993
Title Comparative analysis of internalisation, haemolytic, cytotoxic and antibacterial effect of membrane-active cationic peptides: aspects of experimental setup
Year 2017
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.