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Brevinin-1 CEa
General Information
DCTPep ID DCTPep00211
Peptide Name Brevinin-1 CEa
Sequence FLIGMTQGLICLITRKC
Sequence Length 17
UniProt ID P0C5X0
PubChem CID Not available
Origin skin of the Chinese brown frog, Rana chensinensis
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MCF-7 | Invasive breast carcinoma of no special type | IC50=54 µM | MTT assay | 24 h | 1 |
Hemolytic Activity Human erythrocytes: LD50=48 μM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00211
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C85H148N22O21S3
Absent amino acids ADEHNPSVWY
Theoretical pI 8.96
Acidic residues 0
Basic residues 2
Polar residues 6
Molecular weight (Average) 1910.42
Molecular weight (Monoisotopic) 1909.04
Common amino acids IL
Net charge 2
Instability index (II) 14.61
Aliphatic index 137.65
Grand average of hydropathicity (GRAVY) 1.206
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.065, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 19341344
Title Molecular cloning of cDNAs encoding antimicrobial peptide precursors from the skin of the Chinese brown frog, Rana chensinensis
Year 2009
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.