Brevinin-1 CEa

General Information


DCTPep ID  DCTPep00211

Peptide Name   Brevinin-1 CEa

Sequence  FLIGMTQGLICLITRKC

Sequence Length  17

UniProt ID  P0C5X0 

PubChem CID  Not available

Origin  skin of the Chinese brown frog, Rana chensinensis

Type  Native peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
MCF-7 Invasive breast carcinoma of no special type IC50=54 µM MTT assay 24 h 1

Hemolytic Activity  Human erythrocytes: LD50=48 μM

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00211

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C85H148N22O21S3

Absent amino acids  ADEHNPSVWY

Theoretical pI  8.96

Acidic residues  0

Basic residues  2

Polar residues  6

Molecular weight (Average)  1910.42

Molecular weight (Monoisotopic)  1909.04

Common amino acids  IL

Net charge  2

Instability index (II)  14.61

Aliphatic index  137.65

Grand average of hydropathicity (GRAVY)  1.206

Half Life 
  1.1 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  2 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 125
  Abs 0.1% (=1 g/l) 0.065, assuming all pairs of Cys residues form cystines
  Ext. coefficient 0
  Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 19341344

Title  Molecular cloning of cDNAs encoding antimicrobial peptide precursors from the skin of the Chinese brown frog, Rana chensinensis

Doi 10.2108/zsj.26.220

Year  2009

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.