This work is licensed under a Creative Commons Attribution 4.0 International License.
LfcinB
General Information
DCTPep ID DCTPep00218
Peptide Name LfcinB
Sequence FKCRRWQWRMKK
Sequence Length 12
UniProt ID P24627
PubChem CID Not available
Origin Bovine lactoferrin (Lf-B)
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| Ramos | Burkitt lymphoma; Burkitt lymphoma | IC50=10 µM | MTT assay | 24 h | 1 |
| Raji | EBV-related Burkitt lymphoma; Burkitt lymphoma | IC50=13 µM | MTT assay | 24 h | 1 |
| Kelly | Neuroblastoma | IC50=141 ± 3 µM | MTT assay | 30 min | 2 |
| HT-29 | Colon adenocarcinoma | IC50=148±8 µM | MTT assay | 24 h | 1 |
| SU-DHL-4 | Diffuse large B-cell lymphoma germinal center B-cell type; Diffuse large B-cell lymphoma | IC50=16 µM | MTT assay | 24 h | 1 |
| KMS-5 | Plasma cell myeloma; Multiple myeloma | IC50=38 µM | MTT assay | 24 h | 1 |
| FEMX-I | Melanoma | IC50=40±7 µM | MTT assay | 24 h | 1 |
| U266B1 | Plasma cell myeloma; Multiple myeloma | IC50=55 µM | MTT assay | 24 h | 1 |
| KMM-1 | Plasma cell myeloma; Multiple myeloma | IC50=57 µM | MTT assay | 24 h | 1 |
| MT-1 | Human mammary carcinoma | IC50>160 µM | MTT assay | 30 min | 2 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Peptides induce lysis of cancer cells through interactions with the plasma membrane
Structure Information
PDB ID Not available
Predicted Structure Not available
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C80H125N27O14S2
Absent amino acids ADEGHILNPSTVY
Theoretical pI 11.74
Acidic residues 0
Basic residues 6
Polar residues 1
Molecular weight (Average) 1753.17
Molecular weight (Monoisotopic) 1751.93
Common amino acids KR
Net charge 6
Instability index (II) 92.11
Aliphatic index 0.00
Grand average of hydropathicity (GRAVY) -1.942
Half Life
1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 11000
Abs 0.1% (=1 g/l) 6.274, assuming all pairs of Cys residues form cystines
Ext. coefficient 11000
Abs 0.1% (=1 g/l) 6.274, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 19527490
Title The anticancer activity of lytic peptides is inhibited by heparan sulfate on the surface of the tumor cells
Year 2009
Literature 2
Pubmed ID 21453492
Title Small lytic peptides escape the inhibitory effect of heparan sulfate on the surface of cancer cells
Year 2011
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.