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Latarcin-5
General Information
DCTPep ID DCTPep00227
Peptide Name Latarcin-5
Sequence GFFGKMKEYFKKFGASFKRRFANLKKRL
Sequence Length 28
UniProt ID Not available
PubChem CID Not available
Origin Lachesana tarabaevi
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL1 positive; Chronic myeloid leukemia | EC50=12 µM | Hoechst 33342 and PI assay | 3h | 1 |
Hemolytic Activity Rabbit erythrocytes: 20% Hemolysis=40 µM; Human erythrocytes: 50% Hemolysis=12 µM; 27% Hemolysis=256 µg/ml
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00227
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C164H253N45O34S1
Absent amino acids CDHIPQTVW
Theoretical pI 11.24
Acidic residues 1
Basic residues 10
Polar residues 6
Molecular weight (Average) 3431.15
Molecular weight (Monoisotopic) 3428.92
Common amino acids K
Net charge 9
Instability index (II) 45.2
Aliphatic index 35.00
Grand average of hydropathicity (GRAVY) -0.757
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 1490
Abs 0.1% (=1 g/l) 0.434
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 19563807
Title N-terminal amphipathic helix as a trigger of hemolytic activity in antimicrobial peptides: a case study in latarcins
Doi 10.1016/j.febslet.2009.06.044
Year 2009
Literature 2
Pubmed ID 16735513
Title Latarcins, antimicrobial and cytolytic peptides from the venom of the spider Lachesana tarabaevi (Zodariidae) that exemplify biomolecular diversity
Year 2006
Literature 3
Pubmed ID 34576320
Title Membrane Interactions of Latarcins: Antimicrobial Peptides from Spider Venom
Year 2021
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.