P18

General Information


DCTPep ID  DCTPep00229

Peptide Name   P18

Sequence  KWKLFKKIPKFLHLAKKF

Sequence Length  18

UniProt ID  Not available

PubChem CID  Not available

Origin  Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
A-375 Amelanotic melanoma 86 ± 0.57% cell viability at 10 µM, 63.8 ± 0.8% cell viability at 20 µM, 47.5 ± 1.66% cell viability at 40 µM, 26.9 ± 0.5% cell viability at 60 µM, 6.5 ± 1.07% cell viability at 80 µM MTT assay Not available 1
M14 Amelanotic melanoma 92.2 ± 1.02% cell viability at 10 µM, 71.1 ± 2.84% cell viability at 20 µM, 50 ± 3.09% cell viability at 40 µM, 31.5 ± 1.49% cell viability at 60 µM, 13.8 ± 1.44% cell viability at 80 µM MTT assay Not available 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  99 ± 1.6% cell viability at 10 µM, 98 ± 1.6% cell viability at 20 µM, 75.2 ± 5.6% cell viability at 40 µM, 70.8 ± 3.6% cell viability at 60 µM, 47.7 ± 3.1% cell viability at 80 µM

Target  Not available

Affinity  Not available

Mechanism  Amphiphilic α-helical structure of P18 peptide, coupled with changes in ion channels and an increase in plasma membrane permeability that eventually leads to melanoma cell death



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00229

(Please note that there is the predicted structure, predicted by AlphaFold)

Parsing response... [0/0]
SequenceNo structure available

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C118H186N28O19

Absent amino acids  CDEGMNQRSTVY

Theoretical pI  10.78

Acidic residues  0

Basic residues  8

Polar residues  0

Molecular weight (Average)  2300.95

Molecular weight (Monoisotopic)  2299.44

Common amino acids  K

Net charge  8

Instability index (II)  -9.94

Aliphatic index  92.22

Grand average of hydropathicity (GRAVY)  -0.383

Half Life 
  1.3 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  3 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 5500
  Abs 0.1% (=1 g/l) 2.390

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 19957017

Title  Anti-melanoma activity of hybrid peptide P18 and its mechanism of action

Doi 10.1007/s10529-009-0175-2

Year  2010

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.