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P18
General Information
DCTPep ID DCTPep00229
Peptide Name P18
Sequence KWKLFKKIPKFLHLAKKF
Sequence Length 18
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A-375 | Amelanotic melanoma | 86 ± 0.57% cell viability at 10 µM, 63.8 ± 0.8% cell viability at 20 µM, 47.5 ± 1.66% cell viability at 40 µM, 26.9 ± 0.5% cell viability at 60 µM, 6.5 ± 1.07% cell viability at 80 µM | MTT assay | Not available | 1 |
| M14 | Amelanotic melanoma | 92.2 ± 1.02% cell viability at 10 µM, 71.1 ± 2.84% cell viability at 20 µM, 50 ± 3.09% cell viability at 40 µM, 31.5 ± 1.49% cell viability at 60 µM, 13.8 ± 1.44% cell viability at 80 µM | MTT assay | Not available | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity 99 ± 1.6% cell viability at 10 µM, 98 ± 1.6% cell viability at 20 µM, 75.2 ± 5.6% cell viability at 40 µM, 70.8 ± 3.6% cell viability at 60 µM, 47.7 ± 3.1% cell viability at 80 µM
Target Not available
Affinity Not available
Mechanism Amphiphilic α-helical structure of P18 peptide, coupled with changes in ion channels and an increase in plasma membrane permeability that eventually leads to melanoma cell death
Structure Information
PDB ID Not available
Predicted Structure DCTPep00229
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C118H186N28O19
Absent amino acids CDEGMNQRSTVY
Theoretical pI 10.78
Acidic residues 0
Basic residues 8
Polar residues 0
Molecular weight (Average) 2300.95
Molecular weight (Monoisotopic) 2299.44
Common amino acids K
Net charge 8
Instability index (II) -9.94
Aliphatic index 92.22
Grand average of hydropathicity (GRAVY) -0.383
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 2.390
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 19957017
Title Anti-melanoma activity of hybrid peptide P18 and its mechanism of action
Year 2010
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.