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Camel
General Information
DCTPep ID DCTPep00231
Peptide Name Camel
Sequence KWKLFKKIGAVLKVL
Sequence Length 15
UniProt ID Not available
PubChem CID Not available
Origin Ceropin-Melittin hybrid
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| RenCa | Mouse kidney carcinoma | LC50=1.3 µM | MTT assay | 24 h | 1 |
| B16-F10 | Mouse melanoma | LC50=1.75 µM | MTT assay | 24 h | 1 |
| WM1158 | Melanoma | LC50=4.1 µM | MTT assay | 24 h | 1 |
| 4T1 | Malignant neoplasms of the mouse mammary gland | LC50=6.2 µM | MTT assay | 24 h | 1 |
Hemolytic Activity At CAMEL concentrations toxic for B16-F10 cells the peptide did not cause erythrocyte hemolysis.
Normal (non-cancerous) Cytotoxicity Increasing the amount of CAMEL (1–20 μM) proved toxic to all tested cell lines The LC50 values were from 2.95 to 5.2 μM for normal cells (HECa10, HEC PP, NIH3T3, Wi38)
Target Not available
Affinity Not available
Mechanism specific combinations of necrosis-inducing peptide (CAMEL) and IL-12-carrying plasmid DNA can inhibit tumor growth as well as prevent tumor relapse
Structure Information
PDB ID 2JMY
Predicted Structure DCTPep00231
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C89H151N21O16
Absent amino acids CDEHMNPQRSTY
Theoretical pI 10.60
Acidic residues 0
Basic residues 5
Polar residues 1
Molecular weight (Average) 1771.31
Molecular weight (Monoisotopic) 1770.16
Common amino acids K
Net charge 5
Instability index (II) -28.99
Aliphatic index 149.33
Grand average of hydropathicity (GRAVY) 0.540
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 3.105
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 20212453
Title Anticancer effects of CAMEL peptide
Year 2010
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.