Peptide Libraray

General Information

DCTPep ID DCTPep00233

Peptide Name AP

Sequence ACDCRGDCFCGGGGIVRRADRAAVP

Sequence Length 25

UniProt ID Not available

PubChem CID Not available

Origin Endostatin derived Synthetic

Type Synthetic peptide

Classification

ACP Tumor active peptide Cancer targeted peptides

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
B16-F10	Mouse melanoma	40% inhibition at 5µg/ml	MTT assay	72 h	1
B16-F10	Mouse melanoma	50% inhibition at 2.5µg/ml	MTT assay	72 h	1
B16-F10	Mouse melanoma	75% inhibition at 10µg/ml	MTT assay	72 h	1

Hemolytic Activity Not available

Normal (non-cancerous) Cytotoxicity Not available

Target integrin αvβ3

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00233

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C100H165N37O32S4

Absent amino acids EHKLMNQSTWY

Theoretical pI 7.93

Acidic residues 3

Basic residues 4

Polar residues 9

Molecular weight (Average) 2525.88

Molecular weight (Monoisotopic) 2524.13

Common amino acids G

Net charge 1

Instability index (II) 63.7

Aliphatic index 54.80

Grand average of hydropathicity (GRAVY) 0.032

Half Life
4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 250
Abs 0.1% (=1 g/l) 0.099, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 20515045

Title Effect of RGD-4C Position is More Important Than Disulfide Bonds on Antiangiogenic Activity of RGD-4C Modified Endostatin Derived Synthetic Polypeptide

Doi 10.1021/bc900292y

Year 2010

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available