AP(O)

General Information


DCTPep ID  DCTPep00234

Peptide Name   AP(O)

Sequence  ACDCRGDCFCGGGGIVRRADRAAVP

Sequence Length  25

UniProt ID  Not available

PubChem CID  Not available

Origin  Endostatin derived Synthetic

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Cancer targeted peptides



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
B16-F10 Mouse melanoma 40% inhibition at 2.5µg/ml MTT assay 72 h 1
B16-F10 Mouse melanoma 42% inhibition at 5µg/ml MTT assay 72 h 1
B16-F10 Mouse melanoma 52% inhibition at 10µg/ml MTT assay 72 h 1

Hemolytic Activity  Not available

Normal (non-cancerous) Cytotoxicity  Not available

Target  integrin αvβ3

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00234

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys2 <--> Cys10, Cys4 <--> Cys8

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C100H165N37O32S4

Absent amino acids  EHKLMNQSTWY

Theoretical pI  7.93

Acidic residues  3

Basic residues  4

Polar residues  9

Molecular weight (Average)  2525.88

Molecular weight (Monoisotopic)  2524.13

Common amino acids  G

Net charge  1

Instability index (II)  63.7

Aliphatic index  54.80

Grand average of hydropathicity (GRAVY)  0.032

Half Life 
  4.4 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 250
  Abs 0.1% (=1 g/l) 0.099, assuming all pairs of Cys residues form cystines
  Ext. coefficient 0
  Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 20515045

Title  Effect of RGD-4C Position is More Important Than Disulfide Bonds on Antiangiogenic Activity of RGD-4C Modified Endostatin Derived Synthetic Polypeptide

Doi 10.1021/bc900292y

Year  2010

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.