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AP(O)
General Information
DCTPep ID DCTPep00234
Peptide Name AP(O)
Sequence ACDCRGDCFCGGGGIVRRADRAAVP
Sequence Length 25
UniProt ID Not available
PubChem CID Not available
Origin Endostatin derived Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide Cancer targeted peptides
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| B16-F10 | Mouse melanoma | 40% inhibition at 2.5µg/ml | MTT assay | 72 h | 1 |
| B16-F10 | Mouse melanoma | 42% inhibition at 5µg/ml | MTT assay | 72 h | 1 |
| B16-F10 | Mouse melanoma | 52% inhibition at 10µg/ml | MTT assay | 72 h | 1 |
Hemolytic Activity Not available
Normal (non-cancerous) Cytotoxicity Not available
Target integrin αvβ3
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00234
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys2 <--> Cys10, Cys4 <--> Cys8
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C100H165N37O32S4
Absent amino acids EHKLMNQSTWY
Theoretical pI 7.93
Acidic residues 3
Basic residues 4
Polar residues 9
Molecular weight (Average) 2525.88
Molecular weight (Monoisotopic) 2524.13
Common amino acids G
Net charge 1
Instability index (II) 63.7
Aliphatic index 54.80
Grand average of hydropathicity (GRAVY) 0.032
Half Life
4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 250
Abs 0.1% (=1 g/l) 0.099, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 20515045
Title Effect of RGD-4C Position is More Important Than Disulfide Bonds on Antiangiogenic Activity of RGD-4C Modified Endostatin Derived Synthetic Polypeptide
Year 2010
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.