A20L

General Information


DCTPep ID  DCTPep00254

Peptide Name   A20L

Sequence  KWKSFLKTFKSAKKTVLHTLLKAISS

Sequence Length  26

UniProt ID  Not available

PubChem CID  Not available

Origin  PeptideV13K

Type  Synthetic peptide

Classification

  

ACP Tumor active peptide Membrane-targeted



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
NCI-H1299 Lung large cell carcinoma IC50=2.9 μmol/L MTT assay 36 h 1
HeLa Human papillomavirus-related endocervical adenocarcinoma IC50=4.3 μmol/L MTT assay 36 h 1
SW1116 Colon adenocarcinoma IC50=5 μmol/L MTT assay 36 h 1
MCF-7 Invasive breast carcinoma of no special type IC50=5.1 μmol/L MTT assay 36 h 1
A-375 Amelanotic melanoma IC50=6.5 μmol/L MTT assay 36 h 1
B16 Mouse melanoma IC50=7.8 μmol/L MTT assay 36 h 1
A549 Lung adenocarcinoma IC50=8.7 μmol/L MTT assay 36 h 1
RD Embryonal rhabdomyosarcoma IC50=9.8 μmol/L MTT assay 36 h 1

Hemolytic Activity  Human red blood cells: MHC=41.8 ± 0.22 μmol/L

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Peptides killed cancer cells with a fast necrotic mechanism causing cell membrane lysis



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00254

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Acetylation

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C142H236N36O34

Absent amino acids  CDEGMNPQRY

Theoretical pI  10.78

Acidic residues  0

Basic residues  8

Polar residues  7

Molecular weight (Average)  2991.66

Molecular weight (Monoisotopic)  2989.78

Common amino acids  K

Net charge  8

Instability index (II)  6.56

Aliphatic index  93.85

Grand average of hydropathicity (GRAVY)  -0.138

Half Life 
  1.3 hours (mammalian reticulocytes, in vitro).
  3 min (yeast, in vivo).
  3 min (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 5500
  Abs 0.1% (=1 g/l) 1.838

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 21252288

Title  Studies on mechanism of action of anticancer peptides by modulation of hydrophobicity within a defined structural framework

Doi 10.1158/1535-7163.MCT-10-0811

Year  2011

Literature 2

Pubmed ID 15677462

Title  Rational design of alpha-helical antimicrobial peptides with enhanced activities and specificity/therapeutic index

Doi 10.1074/jbc.M413406200

Year  2005

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.