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A12L/A20L
General Information
DCTPep ID DCTPep00255
Peptide Name A12L/A20L
Sequence KWKSFLKTFKSLKKTVLHTLLKAISS
Sequence Length 26
UniProt ID Not available
PubChem CID Not available
Origin PeptideV13K
Type Synthetic peptide
Classification
ACP Tumor active peptide Membrane-targeted
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=2 μmol/L | MTT assay | 36 h | 1 |
| NCI-H1299 | Lung large cell carcinoma | IC50=4.1 μmol/L | MTT assay | 36 h | 1 |
| SW1116 | Colon adenocarcinoma | IC50=4.3 μmol/L | MTT assay | 36 h | 1 |
| B16 | Mouse melanoma | IC50=4.4 μmol/L | MTT assay | 36 h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=6.2 μmol/L | MTT assay | 36 h | 1 |
| RD | Embryonal rhabdomyosarcoma | IC50=6.4 μmol/L | MTT assay | 36 h | 1 |
| A-375 | Amelanotic melanoma | IC50=8.7 μmol/L | MTT assay | 36 h | 1 |
| A549 | Lung adenocarcinoma | IC50=9.7 μmol/L | MTT assay | 36 h | 1 |
Hemolytic Activity Human red blood cells: MHC=10.5 ± 0.25 μmol/L
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Peptides killed cancer cells with a fast necrotic mechanism causing cell membrane lysis
Structure Information
PDB ID Not available
Predicted Structure DCTPep00255
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Acetylation
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C145H242N36O34
Absent amino acids CDEGMNPQRY
Theoretical pI 10.78
Acidic residues 0
Basic residues 8
Polar residues 7
Molecular weight (Average) 3033.74
Molecular weight (Monoisotopic) 3031.83
Common amino acids K
Net charge 8
Instability index (II) 3.29
Aliphatic index 105.00
Grand average of hydropathicity (GRAVY) -0.062
Half Life
1.3 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
3 min (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.813
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 21252288
Title Studies on mechanism of action of anticancer peptides by modulation of hydrophobicity within a defined structural framework
Doi 10.1158/1535-7163.MCT-10-0811
Year 2011
Literature 2
Pubmed ID 15677462
Title Rational design of alpha-helical antimicrobial peptides with enhanced activities and specificity/therapeutic index
Year 2005
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.