This work is licensed under a Creative Commons Attribution 4.0 International License.
Ranatuerin-2Lb
General Information
DCTPep ID DCTPep00257
Peptide Name Ranatuerin-2Lb
Sequence GILSSIKGVAKGVAKNVAAQLLDTLKCKITGC
Sequence Length 32
UniProt ID Not available
PubChem CID Not available
Origin Rana luteiventris; Rana pretiosa
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| A549 | Lung adenocarcinoma | IC50=15.32 µM | MTT assay | 24h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=37.23 µM | MTT assay | 24h | 1 |
| MCF-7 | Invasive breast carcinoma of no special type | IC50=45.25 µM | MTT assay | 24h | 1 |
| LoVo | Colon adenocarcinoma | IC50=59.78 µM | MTT assay | 24h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis>100 µM; Sheep erythrocytes: <10% Hemolysis=256 µM
Normal (non-cancerous) Cytotoxicity HEK293T: IC50=63.16 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00257
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys27<--->Cys32
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C140H251N39O41S2
Absent amino acids EFHMPRWY
Theoretical pI 9.60
Acidic residues 1
Basic residues 5
Polar residues 11
Molecular weight (Average) 3200.89
Molecular weight (Monoisotopic) 3198.82
Common amino acids K
Net charge 4
Instability index (II) -1.54
Aliphatic index 125.00
Grand average of hydropathicity (GRAVY) 0.591
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.039, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 34073203
Title Prediction of Anticancer Peptides with High Efficacy and Low Toxicity by Hybrid Model Based on 3D Structure of Peptides
Year 2021
Literature 2
Pubmed ID 21295070
Title Host defense peptides in skin secretions of the Oregon spotted frog Rana pretiosa: implications for species resistance to chytridiomycosis
Year 2011
Patent
Patent ID CN113855783A
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.