Cliotides T3, CT3

General Information


DCTPep ID  DCTPep00287

Peptide Name   Cliotides T3, CT3

Sequence  GLPTCGETCTLGTCYVPDCSCSWPICMKN

Sequence Length  29

UniProt ID  C0HKG1  F8W671 

PubChem CID  Not available

Origin  Clitoria ternatea

Type  Native peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HeLa Human papillomavirus-related endocervical adenocarcinoma IC50=2.0 μM MTT assay 72 h 1

Hemolytic Activity  Human red blood cells: HD50=2.0 μM

Normal (non-cancerous) Cytotoxicity  Not available

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  2LAM 

Predicted Structure  DCTPep00287

(Please note that there is the predicted structure, predicted by AlphaFold)

Parsing response... [0/0]
SequenceNo structure available

Helicity  Not available

Linear/Cyclic  Cyclic

Disulfide/Other Bond  Cys5<--->Cys21; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asn29

N-terminal Modification  Free

C-terminal Modification  Free

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C128H200N32O42S7

Absent amino acids  AFHQR

Theoretical pI  4.37

Acidic residues  2

Basic residues  1

Polar residues  17

Molecular weight (Average)  3083.61

Molecular weight (Monoisotopic)  3081.25

Common amino acids  C

Net charge  -1

Instability index (II)  48.53

Aliphatic index  50.34

Grand average of hydropathicity (GRAVY)  0.214

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 7365
  Abs 0.1% (=1 g/l) 2.388, assuming all pairs of Cys residues form cystines
  Ext. coefficient 6990
  Abs 0.1% (=1 g/l) 2.267, assuming all Cys residues are reduced

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 21596752

Title  Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of albumin-1 chain a and cyclotide domains in the Fabaceae family

Doi 10.1074/jbc.M111.229922

Year  2011

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DBAASP ID  DBAASPR_4277

DCTPep is developed by Dr.Zheng's team.