This work is licensed under a Creative Commons Attribution 4.0 International License.
Kalata B1
General Information
DCTPep ID DCTPep00296
Peptide Name Kalata B1
Sequence GLPVCGETCVGGTCNTPGCTCSWPVCTRN
Sequence Length 29
UniProt ID Not available
PubChem CID Not available
Origin Oldenlandia affinis; Viola yedoensis; Viola odorata; Viola baoshanensis
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| K562 | Blast phase chronic myelogenous leukemia, BCR-ABL2 positive; Chronic myeloid leukemia | 50% Cell death=13.1±0.4µM | Resazurin assay | 2, 24h | 3 |
| BGC-823 | Human papillomavirus-related endocervical adenocarcinoma | IC50=1.32±0.15µM | SRB assay | 48h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=10.21±0.43µM | MTT assay | 5h | 1 |
| MM96L | Melanoma | IC50=3.10±0.06µM | MTT assay | 5h | 1 |
| U-937/GTB | Adult acute monocytic leukemia; Acute monoblastic/monocytic leukemia | IC50=6.9µM | FMCA assay | 72h | 2 |
Hemolytic Activity Human erythrocytes: Human erythrocytes: 41% Hemolysis=25 µM; Human erythrocytes: 50% Hemolysis=29.1±1.1 µM
Normal (non-cancerous) Cytotoxicity HFF-1: IC50=2.38±0.09 µM; 50% Cell death=12.0±0.5 µM; CEM-SS cells: IC50=5.7 µM; Human PBMC: 50% Cell death=11.2±0.5 µM
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00296
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys5<--->Cys19; Cys9<--->Cys21; Cys14<--->Cys26; NCB: Gly1<--->Asn29
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C117H187N35O40S6
Absent amino acids ADFHIKMQY
Theoretical pI 5.96
Acidic residues 1
Basic residues 1
Polar residues 19
Molecular weight (Average) 2916.34
Molecular weight (Monoisotopic) 2914.2
Common amino acids C
Net charge 0
Instability index (II) 46.59
Aliphatic index 43.45
Grand average of hydropathicity (GRAVY) 0.152
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5875
Abs 0.1% (=1 g/l) 2.015, assuming all pairs of Cys residues form cystines
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.886, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 21723349
Title Isolation and characterization of cytotoxic cyclotides from Viola philippica
Doi 10.1016/j.peptides.2011.06.016
Year 2011
Literature 2
Pubmed ID 28669767
Title Bactericidal activity of cyclotides where phosphatidylethanolamine-lipid selectivity determines antimicrobial spectra
Doi 10.1016/j.bbamem.2017.06.018
Year 2017
Literature 3
Pubmed ID 25099014
Title Anticancer and toxic properties of cyclotides are dependent on phosphatidylethanolamine phospholipid targeting
Year 2014
Literature 4
Pubmed ID 21576247
Title Decoding the membrane activity of the cyclotide kalata B1: the importance of phosphatidylethanolamine phospholipids and lipid organization on hemolytic and anti-HIV activities
Year 2011
Literature 5
Pubmed ID 18081258
Title Anti-HIV cyclotides from the Chinese medicinal herb Viola yedoensis
Year 2008
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.