Peptide Libraray

NRC-14

General Information

DCTPep ID DCTPep00297

Peptide Name NRC-14

Sequence AGWGSIFKHIFKAGKFIHGAIQAHND

Sequence Length 26

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HL-60	Adult acute myeloid leukemia; Acute myeloid leukemia	50% Cell death=8 µg/ml	MTT assay	2h	1

Hemolytic Activity Human erythrocytes: 50% Hemolysis>256 µg/ml

Normal (non-cancerous) Cytotoxicity Zebrafish embryos: >10% Killing=5 µM (28, 52hpf embryo); 100% Killing=25 µM (4hpf embryo)

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00297

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C134H196N38O32

Absent amino acids CELMPRTVY

Theoretical pI 9.70

Acidic residues 1

Basic residues 6

Polar residues 6

Molecular weight (Average) 2851.27

Molecular weight (Monoisotopic) 2849.49

Common amino acids AGI

Net charge 5

Instability index (II) 15.78

Aliphatic index 75.38

Grand average of hydropathicity (GRAVY) -0.058

Half Life
4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.929

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 21729875

Title The zebrafish embryo as a tool for screening and characterizing pleurocidin host-defense peptides as anti-cancer agents

Doi 10.1242/dmm.007310

Year 2011

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available