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NRC-03
General Information
DCTPep ID DCTPep00304
Peptide Name NRC-03
Sequence GRRKRKWLRRIGKGVKIIGGAALDHL
Sequence Length 26
UniProt ID Not available
PubChem CID Not available
Origin Synthetic
Type Synthetic peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| HL-60 | Adult acute myeloid leukemia; Acute myeloid leukemia | 50% Cell death=18 µg/ml | MTT assay | 2h | 1 |
Hemolytic Activity Human erythrocytes: 50% Hemolysis>256 µg/ml
Normal (non-cancerous) Cytotoxicity Zebrafish embryos: 100% Killing=5 µg/ml (4hpf embryo); 100% Killing=25 µg/ml(28, 52hpf embryo); HUVEC: 50% Cell death=128 µg/ml; HUVEC: 50% Cell death=64 µg/ml
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00304
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Linear
Disulfide/Other Bond Not available
N-terminal Modification Free
C-terminal Modification Amidation
Other Modification None
Chiral L
Physicochemical Information
Formula C132H232N48O29
Absent amino acids CEFMNPQSTY
Theoretical pI 12.19
Acidic residues 1
Basic residues 10
Polar residues 5
Molecular weight (Average) 2955.6
Molecular weight (Monoisotopic) 2953.82
Common amino acids GR
Net charge 9
Instability index (II) 68.95
Aliphatic index 108.85
Grand average of hydropathicity (GRAVY) -0.577
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 1.861
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 21729875
Title The zebrafish embryo as a tool for screening and characterizing pleurocidin host-defense peptides as anti-cancer agents
Year 2011
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.