NRC-06

General Information


DCTPep ID  DCTPep00305

Peptide Name   NRC-06

Sequence  GWGSIFKHGRHAAKHIGHAAVNHYL

Sequence Length  25

UniProt ID  Not available

PubChem CID  Not available

Origin  Pseudopleuronectes americanus

Type  Native peptide

Classification

  

ACP Tumor active peptide



Activity Information


Cell Line Disease Activity Assay Testing Time Literature
HL-60 Adult acute myeloid leukemia; Acute myeloid leukemia 50% Cell death=128 µg/ml MTT assay 2h 1

Hemolytic Activity  Human erythrocytes: 50% Hemolysis>256 µg/ml

Normal (non-cancerous) Cytotoxicity  Zebrafish embryos: 100% Killing=25 µg/ml (4, 28hpf embryo); >10% Killing=5 µg/ml (52hpf embryo)

Target  Not available

Affinity  Not available

Mechanism  Not available



Structure Information


PDB ID  Not available

Predicted Structure  DCTPep00305

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity  Not available

Linear/Cyclic  Linear

Disulfide/Other Bond  Not available

N-terminal Modification  Free

C-terminal Modification  Amidation

Other Modification  None

Chiral  L



Physicochemical Information


Formula  C127H186N42O29

Absent amino acids  CDEMPQT

Theoretical pI  10.29

Acidic residues  0

Basic residues  8

Polar residues  7

Molecular weight (Average)  2765.14

Molecular weight (Monoisotopic)  2763.44

Common amino acids  H

Net charge  8

Instability index (II)  43.08

Aliphatic index  74.40

Grand average of hydropathicity (GRAVY)  -0.376

Half Life 
  30 hours (mammalian reticulocytes, in vitro).
  >20 hours (yeast, in vivo).
  >10 hours (Escherichia coli, in vivo).

Extinction coefficients 
  Ext. coefficient 6990
  Abs 0.1% (=1 g/l) 2.528

Amino acid distribution



Literature Information


Literature 1

Pubmed ID 21729875

Title  The zebrafish embryo as a tool for screening and characterizing pleurocidin host-defense peptides as anti-cancer agents

Doi 10.1242/dmm.007310

Year  2011

Patent

Patent ID Not available

Patent Title  Not available

Other Iinformation  Not available

Other Published ID  Not available




DCTPep is developed by Dr.Zheng's team.