Peptide Libraray

NRC-12

General Information

DCTPep ID DCTPep00306

Peptide Name NRC-12

Sequence GWKKWFNRAKKVGKTVGGLAVDHYL

Sequence Length 25

UniProt ID Not available

PubChem CID Not available

Origin Synthetic

Type Synthetic peptide

Classification

ACP Tumor active peptide

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HL-60	Adult acute myeloid leukemia; Acute myeloid leukemia	50% Cell death=64 µg/ml	MTT assay	2h	1

Hemolytic Activity Human erythrocytes: 50% Hemolysis>256 µg/ml

Normal (non-cancerous) Cytotoxicity Zebrafish embryos: >10% Killing=5 µg/ml (4hpf embryo); >10% Killing=25 µg/ml (28hpf embryo); 100% Killing=5 µg/ml (52hpf embryo)

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00306

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C135H208N38O31

Absent amino acids CEIMPQS

Theoretical pI 10.30

Acidic residues 1

Basic residues 7

Polar residues 7

Molecular weight (Average) 2859.37

Molecular weight (Monoisotopic) 2857.59

Common amino acids K

Net charge 6

Instability index (II) 17.45

Aliphatic index 74.00

Grand average of hydropathicity (GRAVY) -0.520

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 12490
Abs 0.1% (=1 g/l) 4.368

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 21729875

Title The zebrafish embryo as a tool for screening and characterizing pleurocidin host-defense peptides as anti-cancer agents

Doi 10.1242/dmm.007310

Year 2011

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available