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Brevinin-2DYc
General Information
DCTPep ID DCTPep00314
Peptide Name Brevinin-2DYc
Sequence GLFDVVKGVLKGVGKNVAGSLLEQLKCKLSGGC
Sequence Length 33
UniProt ID Not available
PubChem CID Not available
Origin Rana dybowskii; Rana nigromaculata
Type Native peptide
Classification
ACP Tumor active peptide
Activity Information
| Cell Line | Disease | Activity | Assay | Testing Time | Literature |
|---|---|---|---|---|---|
| MCF-7 | Invasive breast carcinoma of no special type | IC50=49.3±7.2 µM | MTT assay | 1h | 1 |
| HeLa | Human papillomavirus-related endocervical adenocarcinoma | IC50=50.2±4.6 µM | MTT assay | 1h | 1 |
Hemolytic Activity Human erythrocytes: 2% Hemolysis=100 µg/ml; 50% Hemolysis=65.2±7.6 µM
Normal (non-cancerous) Cytotoxicity Not available
Target Not available
Affinity Not available
Mechanism Not available
Structure Information
PDB ID Not available
Predicted Structure DCTPep00314
(Please note that there is the predicted structure, predicted by AlphaFold)
Helicity Not available
Linear/Cyclic Cyclic
Disulfide/Other Bond Cys27<--->Cys33
N-terminal Modification Free
C-terminal Modification Free
Other Modification None
Chiral L
Physicochemical Information
Formula C147H254N40O42S2
Absent amino acids HIMPRTWY
Theoretical pI 9.24
Acidic residues 2
Basic residues 5
Polar residues 12
Molecular weight (Average) 3318
Molecular weight (Monoisotopic) 3315.84
Common amino acids G
Net charge 3
Instability index (II) 4.13
Aliphatic index 117.88
Grand average of hydropathicity (GRAVY) 0.470
Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Extinction coefficients
Ext. coefficient 125
Abs 0.1% (=1 g/l) 0.038, assuming all pairs of Cys residues form cystines
Ext. coefficient 0
Abs 0.1% (=1 g/l) 0.000, assuming all Cys residues are reduced
Amino acid distribution
Literature Information
Literature 1
Pubmed ID 23632907
Title Purification, molecular cloning, and antimicrobial activity of peptides from the skin secretion of the black-spotted frog, Rana nigromaculata
Year 2013
Literature 2
Pubmed ID 17698251
Title Purification and characterization of antimicrobial peptides from the skin secretion of Rana dybowskii
Doi 10.1016/j.peptides.2007.07.002
Year 2007
Patent
Patent ID Not available
Patent Title Not available
Other Iinformation Not available
Other Published ID Not available
This work is licensed under a Creative Commons Attribution 4.0 International License.