Peptide Libraray

Cr-ACP1

General Information

DCTPep ID DCTPep00316

Peptide Name Cr-ACP1

Sequence AWKLFDDGV

Sequence Length 9

UniProt ID B3EWE7

PubChem CID Not available

Origin Cycas revoluta seeds

Type Native peptide

Classification

ACP Tumor active peptide Cancer targeted peptides Induce apoptosis

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HCT 15	Colon adenocarcinoma	IC50=0.8 mM	MTT assay	72 h	1
HEp-2	Human papillomavirus-related endocervical adenocarcinoma	IC50=1.5 mM	MTT assay	72 h	1

Hemolytic Activity Human erythrocytes: 5% Hemolysis=1 mM

Normal (non-cancerous) Cytotoxicity NIH 3T3: IC50>4 mM

Target DNA

Affinity Not available

Mechanism Proapoptosis

Structure Information

PDB ID Not available

Predicted Structure Not available

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Free

Other Modification None

Chiral L

Physicochemical Information

Formula C50H71N11O14

Absent amino acids CEHIMNPQRSTY

Theoretical pI 4.21

Acidic residues 2

Basic residues 1

Polar residues 1

Molecular weight (Average) 1050.18

Molecular weight (Monoisotopic) 1049.52

Common amino acids D

Net charge -1

Instability index (II) 13.17

Aliphatic index 86.67

Grand average of hydropathicity (GRAVY) 0.044

Half Life
4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 5500
Abs 0.1% (=1 g/l) 5.237

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 21882228

Title Identification and characterization of a bactericidal and proapoptotic peptide from Cycas revoluta seeds with DNA binding properties

Doi 10.1002/jcb.23343

Year 2012

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available

External Code

DBAASP ID DBAASPR_3238