Peptide Libraray

NRC-07

General Information

DCTPep ID DCTPep00323

Peptide Name NRC-07

Sequence RWGKWFKKATHVGKHVGKAALTAYL

Sequence Length 25

UniProt ID Not available

PubChem CID Not available

Origin Pleurocidin-family

Type Native peptide

Classification

ACP Tumor active peptide Cancer targeted peptides

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HL-60	Adult acute myeloid leukemia; Acute myeloid leukemia	50% Cell death=16 µg/ml	MTT assay	2h	1

Hemolytic Activity 25 μM NRC-07: HMECs Cytotoxicity=20%, Fibroblasts Cytotoxicity=1%, HUVECs Cytotoxicity=17%; 50 μM NRC-07: HMECs Cytotoxicity=47%, Fibroblasts Cytotoxicity=0%, HUVECs Cytotoxicity=17%

Normal (non-cancerous) Cytotoxicity Erythrocytes(25 μM NRC-07): Hemolysis=2%; Erythrocytes(50 μM NRC-07): Hemolysis=3%;

Target Cell surface molecules

Affinity Not available

Mechanism NRC-03- and NRC-07-mediated cell death is initiated by peptide binding to negatively-charged molecules

Structure Information

PDB ID Not available

Predicted Structure DCTPep00323

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Biotinylated

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C136H209N39O29

Absent amino acids CDEIMNPQS

Theoretical pI 10.68

Acidic residues 0

Basic residues 8

Polar residues 6

Molecular weight (Average) 2854.4

Molecular weight (Monoisotopic) 2852.61

Common amino acids K

Net charge 8

Instability index (II) 5.37

Aliphatic index 70.40

Grand average of hydropathicity (GRAVY) -0.404

Half Life
1 hours (mammalian reticulocytes, in vitro).
2 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).

Extinction coefficients
Ext. coefficient 12490
Abs 0.1% (=1 g/l) 4.376

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 22023734

Title Pleurocidin-family cationic antimicrobial peptides are cytolytic for breast carcinoma cells and prevent growth of tumor xenografts

Doi 10.1186/bcr3043

Year 2011

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available