Peptide Libraray

MAC1

General Information

DCTPep ID DCTPep00344

Peptide Name MAC1

Sequence GFGMALKLLKKVL

Sequence Length 13

UniProt ID Not available

PubChem CID 102602730

Origin Macropins and their analogs

Type Synthetic peptide

Classification

ACP Tumor active peptide Membrane-targeted

Activity Information

Cell Line	Disease	Activity	Assay	Testing Time	Literature
HeLa	Human papillomavirus-related endocervical adenocarcinoma	IC50=10±4 µM	MTT assay	48 h	1
CCRF-CEM	Childhood T acute lymphoblastic leukemia; Precursor T-cell acute lymphoblastic leukemia	IC50=12±6 µM	MTT assay	48 h	1
SW480	Colon adenocarcinoma	IC50=23±4 µM	MTT assay	48 h	1

Hemolytic Activity Human erythrocytes: IC50=165 μM

Normal (non-cancerous) Cytotoxicity HUVEC: IC50=28±6 μM

Target Not available

Affinity Not available

Mechanism Not available

Structure Information

PDB ID Not available

Predicted Structure DCTPep00344

(Please note that there is the predicted structure, predicted by AlphaFold)

Helicity Not available

Linear/Cyclic Linear

Disulfide/Other Bond Not available

N-terminal Modification Free

C-terminal Modification Amidation

Other Modification None

Chiral L

Physicochemical Information

Formula C68H120N16O14S1

Absent amino acids CDEHINPQRSTWY

Theoretical pI 10.30

Acidic residues 0

Basic residues 3

Polar residues 2

Molecular weight (Average) 1417.86

Molecular weight (Monoisotopic) 1416.89

Common amino acids L

Net charge 3

Instability index (II) -7.4

Aliphatic index 150.00

Grand average of hydropathicity (GRAVY) 1.031

Half Life
30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Extinction coefficients
Should not be visible by UV spectrophotometry.

Amino acid distribution

Literature Information

Literature 1

Pubmed ID 22100226

Title Toxicity study of antimicrobial peptides from wild bee venom and their analogs toward mammalian normal and cancer cells

Doi 10.1016/j.peptides.2011.11.002

Year 2012

Patent

Patent ID Not available

Patent Title Not available

Other Iinformation Not available

Other Published ID Not available